Vitrification with Glutamine Improves Maturation Rate of Vitrified/Warmed Immature Bovine Oocytes

Autor(es)

Yamada, C. ^[1] ; Feitosa, W. B. ^[1] ; Simoes, R. ^[1] ; Nicacio, A. C. ^[1] ; Mendes, C. M. ^[1] ; Assumpcao, M. E. O. A. ^[1] ; Visintin, J. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anim Reprod, Coll Vet Med & Anim Sci, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 46, n. 1, p. 173-176, FEB 2011.
• Citações Web of Science: 4

Resumo

Contents The current study examined the protective effects of l-glutamine and cytochalasin B during vitrification of immature bovine oocytes. Oocyte vitrification solution (PBS supplemented with 10% FCS, 25% EG, 25% DMSO and 0.5 m trehalose) was the vitrification control. Treatments were the addition of 7 mu g/ml cytochalasin B, 80 mm glutamine or both cytochalasin and glutaminine for 30 s. After warming, oocytes were matured in vitro for 24 h, fixed and stained with Hoechst (33342) for nuclear maturation evaluation. l-glutamine improved the vitrified/warmed immature bovine oocytes viability (32.8%), increasing the nuclear maturation rates compared to other treatments and the no treatment vitrified control (17.4%). There was, however, no effect of cytochalasin B on in vitro maturation (14.4%). (AU)

• Processo FAPESP: 03/08543-1 - Criopreservação de oócitos bovinos pela vitrificação
• Beneficiário: Claudia Yamada
• Modalidade de apoio: Bolsas no Brasil - Mestrado