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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Catalytic ethanolysis of soybean oil with immobilized lipase from Candida antarctica and H-1 NMR and GC quantification of the ethyl esters (biodiesel) produced

Texto completo
Autor(es):
Rosset, Isac G. [1] ; Tavares, Maria Cecilia H. [1] ; Assaf, Elisabete M. [1] ; Porto, Andre Luiz M. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Chem Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: APPLIED CATALYSIS A-GENERAL; v. 392, n. 1-2, p. 136-142, JAN 29 2011.
Citações Web of Science: 36
Resumo

The catalytic ethanolysis of soybean oil with commercial immobilized lipase type B from Candida antarctica to yield ethyl esters (biodiesel) has been investigated. Transesterification was monitored with respect to the following parameters: quantity of biocatalyst, reaction time, amount of water added and turnover of lipase. The highest yields of biodiesel (87% by (1)H NMR; 82.9% by GC) were obtained after a reaction time of 24 h at 32 degrees C in the presence of lipase equivalent to 5.0% (w/w) of the amount of soybean oil present. The production of ethyl esters by enzymatic ethanolysis was not influenced by the addition of water up to 4.0% (v/v) of the alcohol indicating that it is possible to use hydrated ethanol in the production of biodiesel catalyzed by lipase. The immobilized enzyme showed high stability under moderate reaction conditions and retained its activity after five production cycles. The (1)H NMR methodology elaborated for the quantification of biodiesel in unpurified reaction mixtures showed good correlations between the signal areas of peaks associated with the alpha-methylene groups of the ethyl esters and those of the triacyl-glycerides in residual soybean oil. Monoacylglycerides, diacylglycerides and triglycerides could also be detected and quantified in the crude biodiesel using (1)H NMR spectroscopic and GC-FID chromatographic methods. The biodiesel production by enzymatic catalysis was promising. In this case, was produced a low concentration of glycerol (0.74%) and easily removed by water extraction. (C) 2010 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 06/54401-2 - Biocatálise e biotransformação de compostos orgânicos com fungos marinhos, algas marinhas e reações catalisadas com enzimas comerciais
Beneficiário:Andre Luiz Meleiro Porto
Linha de fomento: Auxílio à Pesquisa - Regular