Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis

Autor(es)

Ferreira, Karen S. ^[1] ; Maranhao, Andrea Q. ^[2] ; Garcia, Maria C. C. ^[3] ; Brigido, Marcelo M. ^[2] ; Santos, Suelen S. ^[3] ; Lopes, Jose D. ^[4] ; Almeida, Sandro R. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Biol, Sao Paulo - Brazil

^[2] Univ Brasilia, Dept Biol Celular, Brasilia, DF - Brazil

^[3] Univ Sao Paulo, Dept Anal Clin & Toxicol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 6, n. 1 JAN 7 2011.
• Citações Web of Science: 7

Resumo

Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naive T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model. (AU)

• Processo FAPESP: 07/57594-9 - Producao de sc-fv a partir de anticorpo monocional anti-idiotipico ab2beta que mimetiza o antigeno gp43 de paracoccidioides brasiliensis.
• Beneficiário: Sandro Rogerio de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/50568-2 - Producao de scfv a partir de anticorpo monoclonal anti-idiotipico ab2-beta, quemimetiza o antigeno gp43 de paracoccidioides brasiliensis.
• Beneficiário: Karen Spadari Ferreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado