Binding Events of (S)-N-(3-Oxo-octanoyl)-homoserine Lactone with Agrobacterium tumefaciens Mutant Cells Studied by Saturation Transfer Difference NMR

Autor(es)

Cabeca, Luis Fernando ^[1] ; Pomini, Armando Mateus ^[1] ; Cruz, Pedro Luiz R. ^[1] ; Marsaioli, Anita J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 22, n. 4, p. 702-708, 2011.
• Citações Web of Science: 1

Resumo

Quorum-sensing is a widely studied communication phenomenon in bacteria, which involves the production and detection of signaling substances in relation with cell density and colony behavior. Herein, the membrane binding interactions of the signal (S)-N-(3-oxo-octanoyl)-HSL with A. tumefaciens NTL4(pZLR4) cells were studied using saturation transfer difference NMR spectroscopy (STD-NMR). The substance epitope map was obtained showing that the hydrophobic acyl chain is the most important interacting site for the signal and the cell membrane. Results were interpreted upon comparisons with a simpler system, using liposomes as membrane models. Some insights on the use of β-cyclodextrin as acyl-HSL carrier were also provided. (AU)

• Processo FAPESP: 05/02934-4 - Estudo químico das substâncias sinalizadoras produzidas por bactérias e eucariotos superiores
• Beneficiário: Armando Mateus Pomini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/52389-8 - Caracterizacao de acil-homoserina lactonas produzidas por methylobacterium mesophilicum em meio de cultura contendo glucose.
• Beneficiário: Pedro Luís Rocha da Cruz
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica