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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Binding Events of (S)-N-(3-Oxo-octanoyl)-homoserine Lactone with Agrobacterium tumefaciens Mutant Cells Studied by Saturation Transfer Difference NMR

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Author(s):
Cabeca, Luis Fernando [1] ; Pomini, Armando Mateus [1] ; Cruz, Pedro Luiz R. [1] ; Marsaioli, Anita J. [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 22, n. 4, p. 702-708, 2011.
Web of Science Citations: 1
Abstract

Quorum-sensing is a widely studied communication phenomenon in bacteria, which involves the production and detection of signaling substances in relation with cell density and colony behavior. Herein, the membrane binding interactions of the signal (S)-N-(3-oxo-octanoyl)-HSL with A. tumefaciens NTL4(pZLR4) cells were studied using saturation transfer difference NMR spectroscopy (STD-NMR). The substance epitope map was obtained showing that the hydrophobic acyl chain is the most important interacting site for the signal and the cell membrane. Results were interpreted upon comparisons with a simpler system, using liposomes as membrane models. Some insights on the use of β-cyclodextrin as acyl-HSL carrier were also provided. (AU)

FAPESP's process: 05/02934-4 - Chemical study of signaling substances produced by higher bacteria and eukaryotes
Grantee:Armando Mateus Pomini
Support Opportunities: Scholarships in Brazil - Doctorate