Evaluation of the antibacterial activity of poly-(D,L-lactide-co-glycolide) nanoparticles containing violacein

Autor(es)

Martins, D. ^[1] ; Costa, F. T. M. ^[2] ; Brocchi, M. ^[2] ; Duran, N. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Chem Inst, Biol Chem Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, BR-13083860 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NANOPARTICLE RESEARCH; v. 13, n. 1, p. 355-363, JAN 2011.
• Citações Web of Science: 14

Resumo

Since violacein-an antibiotic, antiviral, and antiparasitic compound-exhibits poor solubility in water, polymeric poly-(D,L-lactide-co-glycolide)nanoparticles containing this compound improved its solubility and biological activity. The nanoparticles were prepared by the nanoprecipitation method and characterized in terms of average diameter, zeta potential, drug loading, polymer recovery, in vitro release kinetic, and in vitro antibacterial activity. Nanoparticles with diameters between 116 and 139 nm and negative-charged outer surfaces were obtained. Drug-loading efficiency and polymer recovery were 87 and 93%, respectively. In vitro release kinetics assays showed that violacein loaded in these nanoparticles has sustained release behavior until 5 days. Both free and nanoparticles-loaded violacein exhibited in vitro antibacterial activity against Staphylococcus aureus ATCC 29213 and ATCC 25923 strains and exhibiting around two to five times lower minimum inhibitory concentration (MIC) than free violacein, respectively. The encapsulated violacein was efficient against methicilin-resistant Staphylococcus aureus (MRSA) strains. No significant activity against Escherichia coli and Salmonella enterica was found. (AU)

• Processo FAPESP: 06/58826-8 - Construção de bactérias recombinantes produtoras de violaceína: possível aplicação no desenvolvimento de novos protocolos de purificação e no desenvolvimento de novos agentes antitumorais
• Beneficiário: Dorival Martins Junior
• Modalidade de apoio: Bolsas no Brasil - Mestrado