Systemic lupus erythematosus exhibits a dynamic and continuum spectrum of effector/regulatory T cells

Autor(es)

Mesquita, Jr., D. ^[1] ; Cruvinel, W. de Melo ^{[2, 1]} ; Araujo, J. A. P. ^[1] ; Pucci, F. V. C. ^[2] ; Salmazi, K. C. ^[3] ; Kallas, E. G. ^[3] ; Andrade, L. E. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Rheumatol, BR-04023062 Sao Paulo - Brazil

^[2] Catholic Univ Goias UCG, Dept Biomed, Goiania, Go - Brazil

^[3] Univ Sao Paulo, Dept Clin Immunol & Allergy, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCANDINAVIAN JOURNAL OF RHEUMATOLOGY; v. 40, n. 1, p. 41-50, JAN-FEB 2011.
• Citações Web of Science: 20

Resumo

Objective: The identification of regulatory T cells (Treg cells) as CD4(+)CD25(high) cells may be upset by the increased frequency of activated effector T cells (Teff cells) in inflammatory diseases such as systemic lupus erythematosus (SLE). This study aimed to evaluate the frequency of T-cell subsets according to the expression of CD25 and CD127 in active (A-SLE) and inactive SLE (I-SLE). Methods: Peripheral blood mononuclear cells (PBMCs) from 26 A-SLE patients (SLE Disease Activity Index (SLEDAI) = 10.17 +/- 3.7), 31 I-SLE patients (SLEDAI = 0), and 26 healthy controls (HC) were analysed by multicolour flow. cytometry. Results: CD25(high) cell frequency was increased in A-SLE (5.2 +/- 5.7%) compared to I-SLE (3.4 +/- 3.4%) and HC (1.73 +/- 0.8%) (p < 0.01). However, the percentage of FoxP3(+) cells in the CD25(high) subset was decreased in A-SLE (24.6 +/- 16.4%) compared to I-SLE (33.7 +/- 16) and HC (45 +/- 25.1%) (p < 0.01). This was partly due to the increased frequency of Teff cells (CD25(high)CD127(+)FoxP3(empty set)) in A-SLE (10.7 +/- 7.3%) compared to I-SLE (8.5 +/- 6.5) and HC (6.1 +/- 1.8%) (p = 0.02). Hence the frequency of Treg cells (CD25(+/high)CD127(low/empty set)FoxP3(+)) was equivalent in A-SLE (1.4 +/- 0.8%), I-SLE (1.37 +/- 1.0%), and HC (1.13 +/- 0.59%) (p = 0.42). A-SLE presented an increased frequency of CD25(+)CD127(+)FoxP3(+) and CD25(empty set)FoxP3(+)CD127(low/empty set) T cells, which may represent intermediate phenotypes between Treg and Teff cells. Conclusions: The present study has provided data supporting normal Treg cell frequency in A-SLE and I-SLE as well as increased frequency of Teff cells in A-SLE. This scenario reflects a Treg/Teff ratio imbalance that may favour the inflammatory phenotype of the disease. In addition, the increased frequency of T cells with putative intermediate phenotypes may be compatible with a highly dynamic immune system in SLE. (AU)

• Processo FAPESP: 07/51349-2 - Avaliação fenotípica das células T regulatórias CD4+CD25+ em pacientes com lúpus erimatoso sistêmico
• Beneficiário: Luiz Eduardo Coelho Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/51934-0 - Lupus eritematoso sistemico: avaliacao in vitro das celulas t regulatorias (cd25+cd4+) frente a estimulo com auto-antigenos.
• Beneficiário: Luiz Eduardo Coelho Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular