Genetic diversity in cultivated carioca common beans based on molecular marker analysis

Autor(es):Mostrar menos -

Kuepper Cardoso Perseguini, Juliana Morini ^[1] ; Chioratto, Alisson Fernando ^[2] ; Zucchi, Maria Imaculada ^[1] ; Colombo, Carlos Augusto ^[1] ; Moraes Carbonell, Sergio Augusto ^[2] ; Costa Mondego, Jorge Mauricio ^[1] ; Gazaffi, Rodrigo ^[3] ; Franco Garcia, Antonio Augusto ^[3] ; de Campos, Tatiana ^{[4, 5]} ; de Souza, Anete Pereira ^{[4, 5]} ; Rubiano, Luciana Benchimol ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron Campinas, Ctr Recursos Genet Vegetais, BR-13012970 Campinas, SP - Brazil

^[2] Inst Agron Campinas, Ctr Graos & Fibras, BR-13012970 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 34, n. 1, p. 88-U263, 2011.
• Citações Web of Science: 18

Resumo

A wide array of molecular markers has been used to investigate the genetic diversity among common bean species. However, the best combination of markers for studying such diversity among common bean cultivars has yet to be determined. Few reports have examined the genetic diversity of the carioca bean, commercially one of the most important common beans in Brazil. In this study, we examined the usefulness of two molecular marker systems (simple sequence repeats - SSRs and amplified fragment length polymorphisms - AFLPs) for assessing the genetic diversity of carioca beans. The amount of information provided by Roger's modified genetic distance was used to analyze SSR data and Jaccards similarity coefficient was used for AFLP data. Seventy SSRs were polymorphic and 20 AFLP primer combinations produced 635 polymorphic bands. Molecular analysis showed that carioca genotypes were quite diverse. AFLPs revealed greater genetic differentiation and variation within the carioca genotypes (Gst = 98% and Fst = 0.83, respectively) than SSRs and provided better resolution for clustering the carioca genotypes. SSRs and AFLPs were both suitable for assessing the genetic diversity of Brazilian carioca genotypes since the number of markers used in each system provided a low coefficient of variation. However, fingerprint profiles were generated faster with AFLPs, making them a better choice for assessing genetic diversity in the carioca germplasm. (AU)

• Processo FAPESP: 06/61848-3 - Estimativa da diversidade genetica entre cultivares do tipo carioca de feijao comum com base em marcadores moleculares.
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/59354-2 - Estimativa da diversidade genética entre cultivares do tipo carioca de feijão comum com base em marcadores moleculares
• Beneficiário: Juliana Morini Küpper Cardoso Perseguini
• Modalidade de apoio: Bolsas no Brasil - Mestrado