Biosynthesis of aphidicolin proceeds via the mevalonate pathway in the endophytic fungus Nigrospora sphaerica

Autor(es)

Lopes, Adriana A. ^[1] ; Pupo, Monica T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 22, n. 1, p. 80-85, 2011.
• Citações Web of Science: 4

Resumo

We have previously identified the endophytic fungus Nigrospora sphaerica as a prolific producer of the bioactive diterpene aphidicolin. Herein we report a study to establish the best conditions for the production of aphidicolin by N. sphaerica in Czapek medium. The sugar source (glucose and sucrose) and the incubation time (4-15 days) were optimized for further application on biosynthetic studies of the diterpene. The highest levels of production of aphidicolin were found on the 8th day with 1%-glucose and on the 12th day with 3%-sucrose based media. The biosynthesis of aphidicolin was investigated using [1-13C]-D-glucose as a precursor, and showed that the isoprene units of aphidicolin are derived from the mevalonate pathway. (AU)

• Processo FAPESP: 08/01220-6 - Estudo biossintético de isoprenóides em Smallanthus sonchifolius e no fungo endofítico Nigrospora sphaerica associado ao monitoramento via LC-MS de culturas de endofíticos na detecção da lactona sesquiterpênica enidrina
• Beneficiário: Adriana Aparecida Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado