Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biosynthesis of aphidicolin proceeds via the mevalonate pathway in the endophytic fungus Nigrospora sphaerica

Full text
Author(s):
Lopes, Adriana A. [1] ; Pupo, Monica T. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of the Brazilian Chemical Society; v. 22, n. 1, p. 80-85, 2011.
Web of Science Citations: 4
Abstract

We have previously identified the endophytic fungus Nigrospora sphaerica as a prolific producer of the bioactive diterpene aphidicolin. Herein we report a study to establish the best conditions for the production of aphidicolin by N. sphaerica in Czapek medium. The sugar source (glucose and sucrose) and the incubation time (4-15 days) were optimized for further application on biosynthetic studies of the diterpene. The highest levels of production of aphidicolin were found on the 8th day with 1%-glucose and on the 12th day with 3%-sucrose based media. The biosynthesis of aphidicolin was investigated using [1-13C]-D-glucose as a precursor, and showed that the isoprene units of aphidicolin are derived from the mevalonate pathway. (AU)

FAPESP's process: 08/01220-6 - Isoprenoid biosynthetic study in Smallanthus sonchifolius and in the endophytic fungus Nigrospora sphaerica associated to the LC-MS monitoring of the sesquiterpene lactone enidrin production in endophytic cultures
Grantee:Adriana Aparecida Lopes
Support Opportunities: Scholarships in Brazil - Post-Doctoral