The Chromosomal Distribution of Microsatellite Repeats in the Genome of the Wolf Fish Hoplias malabaricus, Focusing on the Sex Chromosomes

Autor(es)

Cioffi, M. B. ^[1] ; Kejnovsky, E. ^[2] ; Bertollo, L. A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil

^[2] Acad Sci Czech Republic, Inst Biophys, Lab Plant Dev Genet, CS-61265 Brno - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: Cytogenetic and Genome Research; v. 132, n. 4, p. 289-296, 2011.
• Citações Web of Science: 12
• Assunto(s): Cromossomos sexuais Cromossomo X DNA Peixes

Resumo

Distribution of 12 mono-, di- and tri-nucleotide microsatellites on the chromosomes of 2 karyomorphs with 2 distinct sex chromosome systems (a simple XX/XY - karyomorph B and a multiple X(1)X(1)X(2)X(2)/X(1)X(2)Y - karyomorph D) in Hoplias malabaricus, commonly referred to as wolf fish, was studied using their physical mapping with fluorescence in situ hybridization (FISH). The distribution patterns of different microsatellites along the chromosomes varied considerably. Strong hybridization signals were observed at subtelomeric and heterochromatic regions of several autosomes, with a different accumulation on the sex chromosomes. A massive accumulation was found in the heterochromatic region of the X chromosome of karyomorph B, whereas microsatellites were gathered at centromeres of both X chromosomes as well as in corresponding regions of the neo-Y chromosome in karyomorph D. Our findings are likely in agreement with models that predict the accumulation of repetitive DNA sequences in regions with very low recombination. This process is however in contrast with what was observed in multiple systems, where such a reduction might be facilitated by the chromosomal rearrangements that are directly associated with the origin of these systems. Copyright (C) 2010 S. Karger AG, Basel (AU)

• Processo FAPESP: 09/14881-3 - Evolução cromossômica na família Erythrinidae: mapeamento citogenético de DNAs repetitivos e microdissecção de cromossomos sexuais
• Beneficiário: Marcelo de Bello Cioffi
• Modalidade de apoio: Bolsas no Brasil - Doutorado