Taenia saginata: Production and characterization of monoclonal antibodies against Taenia saginata metacestode antigens

Autor(es)

Vicentini-Oliveira, Josy Campanha ^[1] ; Golim, Marjorie A. ^[2] ; Paulan, Silvana de Cassia ^[3] ; Biondi, Germano Francisco ^[4] ; Rossi-Ferreira, Rosana ^[5] ; Deffune, Elenice ^[6] ; Nunes, Caris Maroni ^[7]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Grad Program Vet Med, Sch Vet Med & Zootechny, BR-18618000 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Flowcitometry Lab, Blood Ctr, Fac Med, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Grad Program Anim Sci, BR-16050680 Aracatuba, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Dept Hyg & Publ Hlth, Sch Vet Med & Zootechny, BR-18618000 Botucatu, SP - Brazil

^[5] Univ Estadual Paulista, UNESP, Dept Biol Sci, Fac Sci, BR-17033360 Bauru, SP - Brazil

^[6] Univ Estadual Paulista, UNESP, Cellular Engn Lab, Blood Ctr, Fac Med, BR-18618970 Botucatu, SP - Brazil

^[7] Univ Estadual Paulista, UNESP, Dept Anim Hlth & Prod, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 126, n. 4, p. 621-625, DEC 2010.
• Citações Web of Science: 4

Resumo

Cysticercosis is a major cause of economic loss in bovine production due to meat condemnation. Chemotherapy is being used in Brazilian cattle and a diagnostic test to improve the treatment program is desired. We produced monoclonal antibodies against crude (TAEB) and cyst fluid (TAEF) Taenia saginata metacestode antigens using immunized BALB/c mice. After cell fusion, 10 TAEB and nine TAEF hybrids were selected and cloned resulting in 18 IgG(1), and 32 IgM TAEB clones, and 9 IgG(1), and 9 IgM TAEF clones. Ascites was produced and Western blot testing was performed resulting in reactivity to protein fractions of low molecular weight (<18 kDa), 43, 55, 66 and 100 kDa. The indirect immunofluorescence test, with one monoclonal antibody against crude and one against cyst fluid antigens, recognized antigenic fractions of both the scolex and the bladder wall of metacestodes from naturally infected bovine. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 08/53723-1 - Produção e caracterização de anticorpos monoclonais contra antígenos de metacestoides de Taenia saginata
• Beneficiário: Caris Maroni Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular