Whole-Genome Characterization of Human and Simian Immunodeficiency Virus Intrahost Diversity by Ultradeep Pyrosequencing

Autor(es):Mostrar menos -

Bimber, Benjamin N. ^[1] ; Dudley, Dawn M. ^[2] ; Lauck, Michael ^[2] ; Becker, Ericka A. ^[1] ; Chin, Emily N. ^[2] ; Lank, Simon M. ^[1] ; Grunenwald, Haiying L. ^[3] ; Caruccio, Nicholas C. ^[3] ; Maffitt, Mark ^[3] ; Wilson, Nancy A. ^[1] ; Reed, Jason S. ^[1] ; Sosman, James M. ^[4] ; Tarosso, Leandro F. ^[5] ; Sanabani, Sabri ^[5] ; Kallas, Esper G. ^[5] ; Hughes, Austin L. ^[6] ; O'Connor, David H. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI 53706 - USA

^[2] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 - USA

^[3] Epictr Biotechnol, Madison, WI 53713 - USA

^[4] Univ Wisconsin, Carbone Canc Ctr, Madison, WI 53705 - USA

^[5] Univ Sao Paulo, Div Clin Immunol & Allergy, Sao Paulo - Brazil

^[6] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Virology; v. 84, n. 22, p. 12087-12092, NOV 2010.
• Citações Web of Science: 48

Resumo

Rapid evolution and high intrahost sequence diversity are hallmarks of human and simian immunodeficiency virus (HIV/SIV) infection. Minor viral variants have important implications for drug resistance, receptor tropism, and immune evasion. Here, we used ultradeep pyrosequencing to sequence complete HIV/SIV genomes, detecting variants present at a frequency as low as 1%. This approach provides a more complete characterization of the viral population than is possible with conventional methods, revealing low-level drug resistance and detecting previously hidden changes in the viral population. While this work applies pyrosequencing to immunodeficiency viruses, this approach could be applied to virtually any viral pathogen. (AU)

• Processo FAPESP: 04/15856-9 - Análise prospectiva das características virológicas e imunológicas em indivíduos com infecção recente pelo HIV-1 das cidades de São Paulo e Santos, SP
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático