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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Whole-Genome Characterization of Human and Simian Immunodeficiency Virus Intrahost Diversity by Ultradeep Pyrosequencing

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Author(s):
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Bimber, Benjamin N. [1] ; Dudley, Dawn M. [2] ; Lauck, Michael [2] ; Becker, Ericka A. [1] ; Chin, Emily N. [2] ; Lank, Simon M. [1] ; Grunenwald, Haiying L. [3] ; Caruccio, Nicholas C. [3] ; Maffitt, Mark [3] ; Wilson, Nancy A. [1] ; Reed, Jason S. [1] ; Sosman, James M. [4] ; Tarosso, Leandro F. [5] ; Sanabani, Sabri [5] ; Kallas, Esper G. [5] ; Hughes, Austin L. [6] ; O'Connor, David H. [1, 2]
Total Authors: 17
Affiliation:
[1] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI 53706 - USA
[2] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 - USA
[3] Epictr Biotechnol, Madison, WI 53713 - USA
[4] Univ Wisconsin, Carbone Canc Ctr, Madison, WI 53705 - USA
[5] Univ Sao Paulo, Div Clin Immunol & Allergy, Sao Paulo - Brazil
[6] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 - USA
Total Affiliations: 6
Document type: Journal article
Source: Journal of Virology; v. 84, n. 22, p. 12087-12092, NOV 2010.
Web of Science Citations: 48
Abstract

Rapid evolution and high intrahost sequence diversity are hallmarks of human and simian immunodeficiency virus (HIV/SIV) infection. Minor viral variants have important implications for drug resistance, receptor tropism, and immune evasion. Here, we used ultradeep pyrosequencing to sequence complete HIV/SIV genomes, detecting variants present at a frequency as low as 1%. This approach provides a more complete characterization of the viral population than is possible with conventional methods, revealing low-level drug resistance and detecting previously hidden changes in the viral population. While this work applies pyrosequencing to immunodeficiency viruses, this approach could be applied to virtually any viral pathogen. (AU)

FAPESP's process: 04/15856-9 - Prospective analysis of the virological and immunological characteristics in individuals with recent HIV-1 infection in the cities of São Paulo and Santos
Grantee:Ricardo Sobhie Diaz
Support Opportunities: Research Projects - Thematic Grants