Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera)

Autor(es)

Fontana, Renato ; Mendes, Maria Anita ^[2] ; Souza, Bibiana Monson de ; Konno, Katsuhiro ; César, Lílian Mari Marcondes ; Malaspina, Osmar ; Palma, Mario Sergio

• Tipo de documento: Artigo Científico
• Fonte: Peptides; v. 25, n. 6, p. 919-928, June 2004.
• Área do conhecimento: Ciências Biológicas - Bioquímica
• Assunto(s): Peptídeos Espectrometria de massas Abelhas Apis mellifera

Resumo

Four antimicrobial peptides were purified from Royal Jelly of honeybees, by using reverse phase-HPLC and sequenced by using Q-Tof-MS/MS: PFKLSLHL-NH2 (Jelleine-I), TPFKLSLHL-NH2 (Jelleine-II), EPFKLSLHL-NH2 (Jelleine-III), and TPFKLSLH-NH2 (Jelleine-IV). The peptides were synthesized on-solid phase, purified and submitted to different biological assays: antimicrobial activity, mast cell degranulating activity and hemolysis. The Jelleines-I-III presented exclusively antimicrobial activities against yeast, Gram+ and Gram- bacteria; meanwhile, Jelleine-IV was not active in none of the assays performed. These peptides do not present any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into Royal Jelly. (AU)

• Processo FAPESP: 01/05060-4 - Caracterizacao estrutural de peptideos biologicamente ativos das peconhas de vespas sociais e solitarias, por espectrometria de massas.
• Beneficiário: Maria Anita Mendes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado