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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Jelleines: a family of antimicrobial peptides from the Royal Jelly of honeybees (Apis mellifera)

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Author(s):
Fontana, Renato ; Mendes, Maria Anita [2] ; Souza, Bibiana Monson de ; Konno, Katsuhiro ; César, Lílian Mari Marcondes ; Malaspina, Osmar ; Palma, Mario Sergio
Total Authors: 7
Document type: Journal article
Source: Peptides; v. 25, n. 6, p. 919-928, June 2004.
Field of knowledge: Biological Sciences - Biochemistry
Abstract

Four antimicrobial peptides were purified from Royal Jelly of honeybees, by using reverse phase-HPLC and sequenced by using Q-Tof-MS/MS: PFKLSLHL-NH2 (Jelleine-I), TPFKLSLHL-NH2 (Jelleine-II), EPFKLSLHL-NH2 (Jelleine-III), and TPFKLSLH-NH2 (Jelleine-IV). The peptides were synthesized on-solid phase, purified and submitted to different biological assays: antimicrobial activity, mast cell degranulating activity and hemolysis. The Jelleines-I-III presented exclusively antimicrobial activities against yeast, Gram+ and Gram- bacteria; meanwhile, Jelleine-IV was not active in none of the assays performed. These peptides do not present any similarity with the other antimicrobial peptides from the honeybees; they are produced constitutively by the workers and secreted into Royal Jelly. (AU)