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Biochemical and immunohistochemistry of sublethal effects thiamethoxam on the Apis mellifera Hymenoptera, apidae) africanized

Grant number: 12/01498-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2012
Effective date (End): September 30, 2015
Field of knowledge:Biological Sciences - Zoology - Applied Zoology
Principal Investigator:Osmar Malaspina
Grantee:Daiana Antonia Tavares
Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated scholarship(s):13/21634-8 - Evaluation of the effects of thiamethoxam on enzymatic activity of Apis mellifera, BE.EP.DR


Honeybees Apis mellifera Africanized species have great economic and ecological importance, because they are pollinators of agricultural crops and natural areas, and produce honey, beeswax, propolis and royal jelly. In recent decades there has been an increase in global agricultural production and consequently the use of insecticides in crop fields was higher, affecting non-target insects such as bees. The thiamethoxam is a neonicotinoid insecticide used to combat insect pests in various crops such as citrus, coffee, and sugar cane. This insecticide acts on the central nervous system of insects, including both adult bees, the larvae through contaminated pollen and nectar. Considering the effects that can be caused by thiamethoxam, this study aims to assess the possible toxic effects of sublethal doses applied in larvae of A. mellifera during all stages of development. After establishing the lethal dose (LD50) of thiamethoxam for larvae, will be adopted DL50/10 sublethal dosages; DL50/20 DL50/50 and that will be used in the experiments of acute intoxication by contact. Will be observed any abnormalities in the developmental stages of larvae and mortalities recorded. After sublethal toxicity bioassay will also be analyzed, survival and longevity. The brain of larvae, pupae and newly emerged workers will be dissected and subjected to confocal microscopic analysis, and enzymatic analysis.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TAVARES, DAIANA ANTONIA; ROAT, THAISA CRISTINA; MATHIAS SILVA-ZACARIN, ELAINE CRISTINA; FERREIRA NOCEELLI, ROBERTA CORNELIO; MALASPINA, OSMAR. Exposure to thiamethoxam during the larval phase affects synapsin levels in the brain of the honey bee. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 169, p. 523-528, . (12/01498-0, 12/13370-8, 12/50197-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TAVARES, Daiana Antonia. Avaliação bioquímica e imunohistoquímica dos efeitos subletais do tiametoxam sobre Apis mellifera (Hymenoptera, Apidae). 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.

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