Pesticides effects on stingless bees (Hymenoptera, Meliponini) imune system

• Grant number: 21/09996-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2021
• End date: September 30, 2023
• Field of knowledge: Biological Sciences - Zoology - Physiology of Recent Groups
• Principal Investigator: Roberta Cornélio Ferreira Nocelli
• Grantee: Cliver Fernandes Farder Gomes
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil
• Associated research grant: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use, AP.BTA.TEM

Abstract

Bees play a fundamental role as pollinators of native and cultivated plants. Among these insects, the Africanized bee Apis mellifera (Hymenoptera: Apidae) is considered an essential pollinator for several crops, mainly due to its generalist habit. However, besides this species, stingless bees deserve attention, as they can pollinate equally or even more efficiently than A. mellifera. Bee populations have been threatened by different factors, including the increased use of pesticides in agriculture that can cause mortality and adverse effects on the morphology of organs. Furthermore, the bee's immune system can be affected after exposure to pesticides, which can directly impact the survival of these pollinators. Apis mellifera is used as a pollinator model in pesticide risk assessment tests for bees in Brazil and other countries. However, considering the ecological and economic importance of stingless bees, toxicity tests for these bees should be carried out to understand whether pesticides affect both bee species in the same manner. In this sense, the project aims to evaluate the interactions and effects of different classes of insecticides on the immune system of two species of native bees and compare them with the Africanized hybrid. Worker bees of the species Melipona scutellaris, Scaptotrigona postica and Africanized A. mellifera from at least three different non-parental colonies will be studied. These bees will be exposed to realistic concentrations (ng/microliter) of a neonicotinoid insecticide (imidacloprid), a fungicide (pyraclostrobin), and a herbicide (glyphosate). For each experiment, groups of 30 bees from three different colonies will be exposed in the laboratory to the sublethal concentrations offered in the food, both in an isolated and combined way. Bees will be collected, anesthetized under refrigeration, and dissected every 24 hours, and the material will be prepared according to specific protocols for each technique used to investigate the effects of sublethal doses of different pesticides in isolated and combined ways on: the morphology of hemocytes and trophocytes through light microscopy and atomic force microscopy; the expression of Heat Shock Proteins (HSPs), autophagy, and cell death by immunohistochemistry, using primary antibodies anti-HSP 70 and 90, anti-LC3 A/B and anti-cleaved caspase 3; the number of immune system cells present in the hemolymph by flow cytometry; the encapsulation rate by the mean gray value per filament; the activity of the enzymes Superoxide Dismutase (SOD), Catalase (CAT), Glutathione S-Transferase (GST) and Phenoloxidase (PO); the expression pattern of defense-related proteins of the organism (abaecin, apidaecin, defensins, glucose dehydrogenase, and hymenoptaecin) by PCR-Real Time; the walking behavior and the mean velocity of the workers through the Ethoflow software. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FARDER-GOMES, CLIVER FERNANDES; BOVO, JOYCE; DA SILVA, JAQUELINE APARECIDA; GONSALVES, POLLYANA LEAO; DE OLIVEIRA, MARCO ANTONIO; NOCELLI, ROBERTA CORNELIO FERREIRA. Larval exposure to thiamethoxam and pyraclostrobin impairs the morphology of the midgut and Malpighian tubules in newly emerged workers of Scaptotrigona postica (Hymenoptera: Meliponini). ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v. 118, p. 8-pg., 2025-09-01. (21/09996-8, 17/21097-3)

ULYSSEA, MONICA A.; FARDER-GOMES, CLIVER F.; PRADO, LIVIA P.. Biological notes, nest architecture, and morphology of the remarkable ant Hylomyrma primavesi ULYSSEA, 2021 (Hymenoptera: Formicidae: Myrmicinae). MYRMECOLOGICAL NEWS, v. 34, p. 20-pg., 2024-01-01. (19/24810-8, 21/09996-8, 18/11453-0, 22/01974-8, 17/21097-3)

FARDER-GOMES, CLIVER FERNANDES; SANTOS, ABRAAO ALMEIDA; FERNANDES, KENNER MORAIS; BERNARDES, RODRIGO CUPERTINO; MARTINS, GUSTAVO FERREIRA; SERRAO, JOSE EDUARDO. Fipronil exposure compromises respiration and damages the Malpighian tubules of the stingless bee Partamona helleri Friese (Hymenoptera: Apidae). Environmental Science and Pollution Research, v. 29, n. 58, p. 8-pg., 2022-07-12. (17/21097-3, 21/09996-8)

FARDER-GOMES, CLIVER FERNANDES; GRELLA, TATIANE CAROLINE; MALASPINA, OSMAR; NOCELLI, ROBERTA FERREIRA CORNELIO. Exposure to sublethal concentrations of imidacloprid, pyraclostrobin, and glyphosate harm the behavior and fat body cells of the stingless bee Scaptotrigona postica. Science of The Total Environment, v. 907, p. 8-pg., 2023-10-28. (17/21097-3, 21/09996-8)

BATISTA, NATHAN RODRIGUES; FARDER-GOMES, CLIVER FERNANDES; NOCELLI, ROBERTA CORNELIO FERREIRA; ANTONIALLI-JUNIOR, WILLIAM FERNANDO. Effects of chronic exposure to sublethal doses of neonicotinoids in the social wasp Polybia paulista: Survival, mobility, and histopathology. Science of The Total Environment, v. 904, p. 12-pg., 2023-12-15. (17/21097-3, 21/09996-8)

DA SILVA, JAQUELINE APARECIDA; FARDER-GOMES, CLIVER FERNANDES; BARCHUK, ANGEL ROBERTO; MALASPINA, OSMAR; NOCELLI, ROBERTA CORNELIO FERREIRA. Sublethal exposure to thiamethoxam and pyraclostrobin affects the midgut and Malpighian tubules of the stingless bee Frieseomelitta varia (Hymenoptera: Apidae: Meliponini). ECOTOXICOLOGY, v. 33, n. 8, p. 9-pg., 2024-07-13. (17/21097-3, 21/09996-8)