Evaluation of the effects of thiamethoxam on enzymatic activity of Apis mellifera

• Grant number: 13/21634-8
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): March 24, 2014
• Effective date (End): November 23, 2014
• Field of knowledge: Biological Sciences - Zoology - Applied Zoology
• Principal researcher: Osmar Malaspina
• Grantee: Daiana Antonia Tavares
• Supervisor abroad: Luc P. Belzunces
• Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Research place: Institut National de la Recherche Agronomique (INRA), France
• Associated to the scholarship: 12/01498-0 - Biochemical and immunohistochemistry of sublethal effects thiamethoxam on the Apis mellifera Hymenoptera, apidae) africanized, BP.DR

Abstract

The neonicotinoid insecticides are widely used in agriculture, and because of being systemic insecticides they are readily translocated within the plant, contaminating pollen and nectar. Thus, in addition to these insecticides act on insect pests, they can contaminate non-target insects such as Apis mellifera. Not only the forage can be exposed to these insecticides, but the larvae that depend on floral resources collected for food. In this context, this work aims to evaluate the effects of sublethal doses of thiamethoxam on the enzyme system of Apis mellifera during larval, pupal and newly emerged stages. For this, will be conducted laboratory bioassays of acute poisoning and continuous, in which the larvae are contaminated via food with sublethal doses of thiamethoxam. At the end of these experiments, brains and intestines will be collected and submitted to analysis of enzyme acetylcholinesterase, carboxylesterase, glutathione-S-transferase and alkaline phosphatase in order to test the possible effect of intoxication on patterns of these enzymes. The data will be statistically reviewed and the results analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TAVARES, DAIANA ANTONIA; DUSSAUBAT, CLAUDIA; KRETZSCHMAR, ANDRE; CARVALHO, STEPHAN MALFITANO; SILVA-ZACARIN, ELAINE C. M.; MALASPINA, OSMAR; BERAIL, GERALDINE; BRUNET, JEAN-LUC; BELZUNCES, LUC P. Exposure of larvae to thiamethoxam affects the survival and physiology of the honey bee at post-embryonic stages. Environmental Pollution, v. 229, p. 386-393, OCT 2017. Web of Science Citations: 11.