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A neuroproteomic approach of the brain from Africanized Apis mellifera submitted to the assay proboscis extension reflex

Grant number: 13/13542-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2013
Effective date (End): June 30, 2017
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal researcher:Mario Sergio Palma
Grantee:Anally Ribeiro da Silva Menegasso
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated research grant:11/51684-1 - System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State, AP.BTA.TEM
Associated scholarship(s):15/26025-5 - Identification of membrane proteins in Apis mellifera brain submitted to the proboscis extension reflex assay, BE.EP.DR


Apis mellifera is an organism characterized by having a nervous system structured, with about 950.000 neurons, but have a wealth behavioral and impressive cognitive ability. Currently, the bees have been used as models of learning and memory, highlighting its usefulness for neuroscience, in particular to a better understand the foundations of cognition. For applications in proteomics and peptidomics analysis, bees are good targets because they have their genome sequenced. However, much remains to decipher at molecular level in terms of learning and memory formation in honeybees. In the present study the behavior of "Proboscis Extension Reflex" (PER) is used as input reference for neuroproteomic studies of the bee brain. For this approach to following experimental strategies will be used: I) shotgun proteomics LC-MS/IT-ToF system for analysis of soluble proteins; II) the use of LC-IT-ETD system for analysis of membrane proteins/receptors isolated by BN-PAGE both for proteins identifications, and PTMs assignment. The proteins identified will be quantified by mass spectrometry using the label-free strategy, in order to understand the complex neural network and the memory which the bees will be subjected; III) MALDI Imaging strategy will also be applied to proteins identified by mass spectrometry and some neuropeptides already described in the literature, to understand the distribution of these macromolecules in different brain structures, collaborating with understanding the biological question of this study. The use of different methodological strategies implemented is expected to contribute to the understanding of neuroscience at the level of proteins and peptides and their role in molecular interactions and in the formation of short-term memory in bees. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROAT, THAISA C.; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; MIOTELO, LUCAS; DE SOUZA, CAROLINE LACERRA; PALMA, MARIO SERGIO; MALASPINA, OSMAR. Using a toxicoproteomic approach to investigate the effects of thiamethoxam into the brain of Apis mellifera. Chemosphere, v. 258, . (14/05376-1, 13/13542-6, 19/04538-1, 12/13370-8, 13/26451-9, 16/16212-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MENEGASSO, Anally Ribeiro da Silva. A neuroprpteomic approach on worker africanized honeybee Apis mellifera submitted to proboscis extension reflex assay. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.

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