Rapid Determination of Water-Soluble and Fat-Soluble Vitamins in Commercial Formulations by MEEKC

Autor(es)

Aurora-Prado, Maria S. ^[1] ; Silva, Claudinei A. ^[2] ; Tavares, Marina F. M. ^[2] ; Altria, Kevin D. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, BR-05508000 Sao Paulo - Brazil

^[3] GlaxoSmithKline Pharmaceut Dev, Harlow CM19 5AW, Essex - England

• Tipo de documento: Artigo Científico
• Fonte: Chromatographia; v. 72, n. 7-8, p. 687-694, OCT 2010.
• Citações Web of Science: 15

Resumo

Microemulsion electrokinetic capillary chromatography has been successfully applied to the separation and determination of water-soluble vitamins (thiamine hydrochloride, riboflavin, niacin, pyridoxine hydrochloride, folic acid, cobalamin, ascorbic acid) and a fat-soluble vitamin (alpha-tocopherol acetate). The optimal microemulsion buffer contained sodium dodecylsulfate (SDS) as surfactant, butan-1-ol as the co-surfactant, ethyl acetate as the oil and pH 9.2 tetraborate buffer, modified with 15% (v/v) 2-propanol. UV detection at 214 nm gave adequate sensitivity without interference from sample excipients. Under the optimized conditions, the vitamins were baseline separated in less than 7 min. Analytical curves of peak area versus concentration presented coefficients of determination (R (2) ) > 0.99, acceptable limits of quantification between 8.40 and 16.23 mu g mL(-1) were obtained. Vitamin levels in liquid formulation were quantified with intra-day precision better than 0.99% RSD for migration time and 1.19% RSD for peak area ratio. Recoveries ranged between 98.7 and 101.7%. The method was considered appropriate for rapid and routine analysis. (AU)

• Processo FAPESP: 00/04414-4 - Técnicas miniaturizadas de separação e análise aplicadas a problemas de impacto clínico/forense, cosmiátrico, ambiental e alimentos
• Beneficiário: Marina Franco Maggi Tavares
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/07390-4 - Validação cruzada de métodos eletroforéticos e cromatográficos em microemulsão para a determinação de vitaminas lipo e hidrossolúveis
• Beneficiário: María Segunda Aurora Prado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado