Morpho-physical Recording of Bovine Conceptus (Bos indicus) and Placenta from Days 20 to 70 of Pregnancy

Autor(es)

Assis Neto, A. C. ^[1] ; Pereira, F. T. V. ^[1] ; Santos, T. C. ^[2] ; Ambrosio, C. E. ^[3] ; Leiser, R. ^[4] ; Miglino, M. A. ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, BR-17900000 Sao Paulo - Brazil

^[2] Univ Estadual Maringa, Dept Anim Sci, Maringa, Parana - Brazil

^[3] Univ Sao Paulo, Dept Surg, Sch Vet Med & Anim Sci, Sao Paulo - Brazil

^[4] Univ Giessen, Dept Vet Anat Histol & Embryol, Giessen - Germany

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 45, n. 5, p. 760-772, OCT 2010.
• Citações Web of Science: 42

Resumo

The study is based on 141 pregnant Bos indicus cows, from days 20 to 70 post-insemination. First, special attention was given to the macroscopically observable phenomena of attachment of the conceptus to the uterus, i.e. the implantation, from about days 20 to 30 post-insemination up to day 70, and placentome development by growth, vascularization and increase in the number of cotyledons opposite to the endometrial caruncles. Secondly, as for the conceptuses, semiquantitative, statistical analyses were performed of the lengths of chorio-allantois, amnion and yolk sac; and the different parts of the centre and two extremes of the yolk sacs were also analysed. Thirdly, the embryos/foetuses corresponding to their membranes were measured by their greatest length and by weight, and described by the appearance of external developmental phenomena during the investigated period like neurulation, somites, branchial arcs, brain vesicles, limb buds, C-form, pigmented eye and facial grooves. In conclusion, all the data collected in this study from days 20 to 70 of bovine pregnancy were compared extensively with corresponding data of the literature. This resulted in an `embryo/foetal age-scale', which has extended the data in the literature by covering the first 8 to 70 days of pregnancy. This age-scale of early bovine intrauterine development provides model for studies, even when using slaughtered cows without distinct knowledge of insemination or fertilization time, through macroscopic techniques. This distinctly facilitates research into the cow, which is now being widely used as `an experimental animal' for testing new techniques of reproduction like in vitro fertilization, embryo transfer and cloning. (AU)

• Processo FAPESP: 06/53009-1 - Mecanismos de falhas na inicialização do parto em conceptos bovinos obtidos por transferência de núcleo de células somáticas (tncs) e associação com anormalidades pré e pós natais
• Beneficiário: Antônio Chaves de Assis Neto
• Modalidade de apoio: Bolsas no Exterior - Novas Fronteiras
• Processo FAPESP: 02/00233-0 - Reprodução e produção animal: o dialogo materno-fetal em ruminantes e seus reflexos na economia agropecuária brasileira
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Temático