Development and characterization of microsatellite markers for turmeric (Curcuma longa)

Autor(es)

Sigrist, M. S. ^[1] ; Pinheiro, J. B. ^[2] ; Azevedo-Filho, J. A. ^[3] ; Colombo, C. A. ^[1] ; Bajay, M. M. ^[2] ; Lima, P. F. ^[1] ; Camilo, F. R. ^[1] ; Sandhu, S. ; Souza, A. P. ^{[4, 5]} ; Zucchi, M. I. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron Campinas, Ctr Pesquisa & Desenvolvimento Recursos Genet, BR-13075630 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Dept Genet, BR-13400970 Piracicaba, SP - Brazil

^[3] Estrada Vicinal Nelson Tauf Nacif, BR-13910000 Monte Alegre Do Sul, SP - Brazil

^[4] Peoples Grp, Ctr Sci Res & Dev, Bhopal 462037, MP - India

^[5] Univ Campinas UNICAMP, Dept Genet & Evolucao, Inst Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT BREEDING; v. 129, n. 5, p. 570-573, OCT 2010.
• Citações Web of Science: 12

Resumo

P>Curcuma longa L. is a sterile, triploid, vegetatively-propagated crop cultivated mainly in Southeast Asia. When dried rhizomes are ground, the resulting yellow powder is used by the food industry as a natural food dye. Moreover, many pharmacological compounds have broadened the commercial application of the crop. However, conventional breeding is difficult and hence, improvement has been limited to germplasm selection. To better utilize the germplasm collections and facilitate genotype selection, a total of 17 polymorphic microsatellite loci were developed using a CT/GT/CTT enriched genomic library. All microsatellites resulted in amplified PCR products, showing a banding pattern of 2-11 polymorphic bands per locus, enabling genotype discrimination. These results can be used in further studies aimed at characterizing C. longa genetic resource collections and also to improve breeding strategies. (AU)

• Processo FAPESP: 07/02895-4 - Caracterização e avaliação de germoplasma de açafrão (Curcuma longa L.) utilizando marcadores morfométricos e microssatélites
• Beneficiário: Maria Imaculada Zucchi
• Modalidade de apoio: Auxílio à Pesquisa - Regular