| Texto completo | |
| Autor(es): |
Lima, Paula M.
[1]
;
Neves, Renato de C. F.
[1]
;
dos Santos, Felipe A.
[1]
;
Perez, Carlos A.
[2]
;
da Silva, Marcelo O. A.
[3, 4]
;
Arruda, Marco A. Z.
[3, 4]
;
de Castro, Gustavo R.
[5]
;
Padilha, Pedro M.
[5]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] UNESP, Fac Med Vet & Zootecn, Programa Posgrad Zootecn, BR-15385000 Botucatu, SP - Brazil
[2] Lab Nacl Luz Sincronton, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13084971 Campinas, SP - Brazil
[4] Univ Estadual Campinas, Inst Quim, GEPAM, BR-13084971 Campinas, SP - Brazil
[5] UNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618970 Botucatu, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Talanta; v. 82, n. 3, p. 1052-1056, AUG 15 2010. |
| Citações Web of Science: | 22 |
| Resumo | |
An investigation was made into calcium, iron and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (20-PAGE) and subsequent qualitative and quantitative evaluation by synchrotron radiation X-ray fluorescence (SRXRF) and Flame Atomic Absorption Spectrometry (FAAS). An analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver protein spots, respectively. The metal ions found were distributed mainly in proteins with a molar mass of less than 40.00 kDa and more than 12.00 kDa, with pI in the range of 4.70-9.40. The only exception was a spot presenting protein with a molar mass of 10.10 kDa. In addition to calcium, iron and zinc, sulfur and phosphorus - which are non-metals that may be part of the protein structure, were also detected. After microwave-assisted acid mineralization of the proteins spots, a FAAS estimation of the concentration of calcium, iron and zinc ions bound to these proteins indicated a range of 1.08-5.80 mg g(-1), 2.02-8.03 mg g(-1) and 1.60-8.55 mg g(-1), respectively. (C) 2010 Published by Elsevier B.V. (AU) | |
| Processo FAPESP: | 07/59778-0 - Desenvolvimento de metodologias analiticas para avaliacao de metaloproteinas de tilapia do nilo (oreochromis niloticus). |
| Beneficiário: | Pedro de Magalhães Padilha |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 03/13362-6 - Determinacao de ca, cu, cr, fe, mn, se e zn em suspensoes de racoes e fezes de peixes por espectrometria de absorcao atomica em forno de grafite para estimativa da biodisponibilidade relativa de... |
| Beneficiário: | Pedro de Magalhães Padilha |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |