Analytical approach to the metallomic of Nile tilapia (Oreochromis niloticus) liver tissue by SRXRF and FAAS after 2D-PAGE separation: Preliminary results

Autor(es)

Lima, Paula M. ^[1] ; Neves, Renato de C. F. ^[1] ; dos Santos, Felipe A. ^[1] ; Perez, Carlos A. ^[2] ; da Silva, Marcelo O. A. ^{[3, 4]} ; Arruda, Marco A. Z. ^{[3, 4]} ; de Castro, Gustavo R. ^[5] ; Padilha, Pedro M. ^[5]

Afiliação do(s) autor(es)

^[1] UNESP, Fac Med Vet & Zootecn, Programa Posgrad Zootecn, BR-15385000 Botucatu, SP - Brazil

^[2] Lab Nacl Luz Sincronton, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13084971 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Inst Quim, GEPAM, BR-13084971 Campinas, SP - Brazil

^[5] UNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 82, n. 3, p. 1052-1056, AUG 15 2010.
• Citações Web of Science: 22

Resumo

An investigation was made into calcium, iron and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (20-PAGE) and subsequent qualitative and quantitative evaluation by synchrotron radiation X-ray fluorescence (SRXRF) and Flame Atomic Absorption Spectrometry (FAAS). An analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver protein spots, respectively. The metal ions found were distributed mainly in proteins with a molar mass of less than 40.00 kDa and more than 12.00 kDa, with pI in the range of 4.70-9.40. The only exception was a spot presenting protein with a molar mass of 10.10 kDa. In addition to calcium, iron and zinc, sulfur and phosphorus - which are non-metals that may be part of the protein structure, were also detected. After microwave-assisted acid mineralization of the proteins spots, a FAAS estimation of the concentration of calcium, iron and zinc ions bound to these proteins indicated a range of 1.08-5.80 mg g(-1), 2.02-8.03 mg g(-1) and 1.60-8.55 mg g(-1), respectively. (C) 2010 Published by Elsevier B.V. (AU)

• Processo FAPESP: 07/59778-0 - Desenvolvimento de metodologias analíticas para avaliação de metaloproteínas de tilápia do Nilo (Oreochromis niloticus)
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/13362-6 - Determinação de Ca, Cu, Cr, Fe, Mn, Se e Zn em suspensões de rações e fezes de peixes por espectrometria de absorção atômica em forno de grafite para estimativa da biodisponibilidade relativa de nutrientes minerais utilizados em piscicultura
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular