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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analytical approach to the metallomic of Nile tilapia (Oreochromis niloticus) liver tissue by SRXRF and FAAS after 2D-PAGE separation: Preliminary results

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Author(s):
Lima, Paula M. [1] ; Neves, Renato de C. F. [1] ; dos Santos, Felipe A. [1] ; Perez, Carlos A. [2] ; da Silva, Marcelo O. A. [3, 4] ; Arruda, Marco A. Z. [4, 3] ; de Castro, Gustavo R. [5] ; Padilha, Pedro M. [5]
Total Authors: 8
Affiliation:
[1] UNESP, Fac Med Vet & Zootecn, Programa Posgrad Zootecn, BR-15385000 Botucatu, SP - Brazil
[2] Lab Nacl Luz Sincronton, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Inst Quim, GEPAM, BR-13084971 Campinas, SP - Brazil
[4] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13084971 Campinas, SP - Brazil
[5] UNESP, Inst Biociencias, Dept Quim & Bioquim, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Talanta; v. 82, n. 3, p. 1052-1056, AUG 15 2010.
Web of Science Citations: 22
Abstract

An investigation was made into calcium, iron and zinc in protein spots in samples of Nile tilapia (Oreochromis niloticus) liver tissue obtained after protein separation by two-dimensional polyacrylamide gel electrophoresis (20-PAGE) and subsequent qualitative and quantitative evaluation by synchrotron radiation X-ray fluorescence (SRXRF) and Flame Atomic Absorption Spectrometry (FAAS). An analysis of the fluorescence spectra indicated the presence of calcium, iron and zinc in 12, 6 and 8 liver protein spots, respectively. The metal ions found were distributed mainly in proteins with a molar mass of less than 40.00 kDa and more than 12.00 kDa, with pI in the range of 4.70-9.40. The only exception was a spot presenting protein with a molar mass of 10.10 kDa. In addition to calcium, iron and zinc, sulfur and phosphorus - which are non-metals that may be part of the protein structure, were also detected. After microwave-assisted acid mineralization of the proteins spots, a FAAS estimation of the concentration of calcium, iron and zinc ions bound to these proteins indicated a range of 1.08-5.80 mg g(-1), 2.02-8.03 mg g(-1) and 1.60-8.55 mg g(-1), respectively. (C) 2010 Published by Elsevier B.V. (AU)

FAPESP's process: 07/59778-0 - Development of analytical methods for evaluation of Nile tilapia (Oreochromis niloticus) metalloproteins
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants