Single Session to Infrared Low Level Diode Laser on TNF-alpha. and IL-6 Cytokines Release by Mononuclear Spleen Cells in Mice: A Pilot Study

Autor(es)

Fukuda, Thiago Y. ^[1] ; Tanji, Maury M. ^{[2, 3]} ; Jesus, Julio F. ^[1] ; Sato, Maria N. ^[2] ; Duarte, Alberto J. S. ^[2] ; Plapler, Helio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, BR-04023062 Sao Paulo - Brazil

^[2] FMUSP, Lab Med Invest Dermatol & Immunodeficiencies, LIM 56, Sao Paulo - Brazil

^[3] Univ Grande ABC, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Surgery and Medicine; v. 42, n. 6, p. 584-588, AUG 2010.
• Citações Web of Science: 10

Resumo

Background and Objective: The results of low-level infrared laser (LLL) systemic action on inflammatory modulation process, specifically diminishing pro-inflammatory and producing anti-inflammatory cytokines are extremely controversial in the literature. More studies are necessary to clarify the biomodulation process The main objective was to investigate the effect of a single session of an AsGaAl laser on spleen cells interleukin-6 (IL-6) and tumor necrosis factor - alpha (TNF-alpha) release, in vivo, in mice Study Design/Materials and Methods: In a pilot study, 18 isogenic mice were distributed in three groups: control (no surgical procedure, n = 6), sham (surgical procedure with three standard cutaneous incisions, followed by abdominal muscle incision followed by suture, n = 6) and LLL (same procedure followed by a single LLL exposure 12 hours after the procedure, n = 6) The animals in the LLL group received a single infrared continuous laser session (780 nm wavelength, power of 20 mW, energy density of 10J/cm(2)) on three points (20 seconds per point), and final energy of 0 4J. All animals of the sham and LLL groups were sacrificed 36 hours after surgical procedure; the spleen mononuclear cells were isolated and cultivated for 48 hours The IL-6 and TNF-a were measured by the ELISA method. Results: IL-6 and TNF-a concentrations released by the mononuclear cells showed significant differences between the control and sham group (P<007) However, there were no differences between the control and LLL group and between the sham and LLL groups (P>007) Conclusion: The single session of infrared LLL showed a tendency of decreasing the IL-6 and TNF-a release by mononuclear spleen cells in mice after application, although there was not a significant difference between the sham and LLL group Conclusions regarding effectiveness of a single session procedure cannot be made due to the low statistical power of this pilot study. Lasers Surg Med 42 584-588, 2010 (C) 2010 Wiley-Liss, Inc (AU)

• Processo FAPESP: 07/04479-8 - Modulação de processos imunológicos in vivo sob ação do laser de baixa potência: fatores pró e antiinflamatórios
• Beneficiário: Helio Plapler
• Modalidade de apoio: Auxílio à Pesquisa - Regular