| Full text | |
| Author(s): |
Fukuda, Thiago Y.
[1]
;
Tanji, Maury M.
[2, 3]
;
Jesus, Julio F.
[1]
;
Sato, Maria N.
[2]
;
Duarte, Alberto J. S.
[2]
;
Plapler, Helio
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, BR-04023062 Sao Paulo - Brazil
[2] FMUSP, Lab Med Invest Dermatol & Immunodeficiencies, LIM 56, Sao Paulo - Brazil
[3] Univ Grande ABC, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Lasers in Surgery and Medicine; v. 42, n. 6, p. 584-588, AUG 2010. |
| Web of Science Citations: | 10 |
| Abstract | |
Background and Objective: The results of low-level infrared laser (LLL) systemic action on inflammatory modulation process, specifically diminishing pro-inflammatory and producing anti-inflammatory cytokines are extremely controversial in the literature. More studies are necessary to clarify the biomodulation process The main objective was to investigate the effect of a single session of an AsGaAl laser on spleen cells interleukin-6 (IL-6) and tumor necrosis factor - alpha (TNF-alpha) release, in vivo, in mice Study Design/Materials and Methods: In a pilot study, 18 isogenic mice were distributed in three groups: control (no surgical procedure, n = 6), sham (surgical procedure with three standard cutaneous incisions, followed by abdominal muscle incision followed by suture, n = 6) and LLL (same procedure followed by a single LLL exposure 12 hours after the procedure, n = 6) The animals in the LLL group received a single infrared continuous laser session (780 nm wavelength, power of 20 mW, energy density of 10J/cm(2)) on three points (20 seconds per point), and final energy of 0 4J. All animals of the sham and LLL groups were sacrificed 36 hours after surgical procedure; the spleen mononuclear cells were isolated and cultivated for 48 hours The IL-6 and TNF-a were measured by the ELISA method. Results: IL-6 and TNF-a concentrations released by the mononuclear cells showed significant differences between the control and sham group (P<007) However, there were no differences between the control and LLL group and between the sham and LLL groups (P>007) Conclusion: The single session of infrared LLL showed a tendency of decreasing the IL-6 and TNF-a release by mononuclear spleen cells in mice after application, although there was not a significant difference between the sham and LLL group Conclusions regarding effectiveness of a single session procedure cannot be made due to the low statistical power of this pilot study. Lasers Surg Med 42 584-588, 2010 (C) 2010 Wiley-Liss, Inc (AU) | |
| FAPESP's process: | 07/04479-8 - In vivo immunological processes modulation with low level laser therapy: pro and anti-inflammatory factors |
| Grantee: | Helio Plapler |
| Support Opportunities: | Regular Research Grants |