Treating metabolic syndrome's metaflammation with low level light therapy

• Grant number: 14/02564-1
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2015 - February 28, 2018
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Martha Simões Ribeiro
• Grantee: Martha Simões Ribeiro
• Host Institution: Instituto de Pesquisas Energéticas e Nucleares (IPEN). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Metabolic syndrome (MS) is a constellation of morbidities comprising dysglycemia, high blood pressure, atherogenic dyslipidemia, and obesity (especially abdominal). This clinical condition increases the risk of developing cardiovascular disease (CVD) and/or diabetes mellitus (DM). Obese individuals present an inflammatory infiltrate among adipocytes from their abdominal adipose tissue. These inflammatory cells are responsible for producing cytokines and proinflammatory chemokines. This inflammatory state, known as metaflammation, is closely related to the establishment and progression of MS by inhibiting intracellular insulin signaling in insulin-dependent tissues. In obese mice, these features can be also observed, validating the use of this animal model for the development of therapeutic approaches to MS. C57BL/6 mice will receive hypercaloric, hyperlipidic and fructose enriched diet during eight weeks. Experimental groups will be transcutaneously irradiated on the abdominal area with a LED (light-emitting diode) dispositive in the near infrared wavelength (» = 850 nm), with different fluence rates. During the trial period, glucose, total cholesterol, triglycerides and proinflammatory cytokines plasma concentrations will be evaluated. The animals will be euthanized by cervical dislocation after anesthetic overdose at different times during the experiment, for intra-abdominal adipose tissue sample collection for histological and immunohistochemical purposes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PRETTO, LUCAS RAMOS; YOSHIMURA, TANIA MATEUS; RIBEIRO, MARTHA SIMOES; DE FREITAS, ANDERSON ZANARDI. Optical coherence tomography for blood glucose monitoring in vitro through spatial and temporal approaches. Journal of Biomedical Optics, v. 21, n. 8, AUG 2016. (13/09311-9, 13/05492-9, 14/02564-1)

YOSHIMURA, TANIA MATEUS; SABINO, CAETANO PADIAL; RIBEIRO, MARTHA SIMOES. Photobiomodulation reduces abdominal adipose tissue inflammatory infiltrate of diet-induced obese and hyperglycemic mice. Journal of Biophotonics, v. 9, n. 11-12, SI, p. 1255-1262, DEC 2016. (14/02564-1)

SABINO, CAETANO P.; DEANA, ALESSANDRO M.; YOSHIMURA, TANIA M.; DA SILVA, DANIELA F. T.; FRANCA, CRISTIANE M.; HAMBLIN, MICHAEL R.; RIBEIRO, MARTHA S.. The optical properties of mouse skin in the visible and near infrared spectral regions. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 160, p. 72-78, JUL 2016. (14/02564-1)