Action of nicotinic acid in adiponectin, leptin and PAI-1 production and in PPAR gamma and PPAR alpha expression in 3T3-L1 adipocytes under oxigen deprivation

• Grant number: 11/07248-2
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2011
• End date: August 31, 2013
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Marcelo Costa Batista
• Grantee: Renata Nakamichi
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Cardiovascular Diseases (CVD) have been considered a major cause of mortality. The independent risk factors of CVD are Dyslipidemia, Sedentary Lifestyle, Smoking, Diabetes Mellitus, Chronic Inflammation, Atherosclerosis and Obesity. Among the risk factors associated with cardiovascular disease, Obesity a worldwide epidemic disease, has been considered one of the major contributors to the current global mortality and morbidity. A expansion of adipose tissue in obesity leads an increased expression of some inflammatory proteins, including TNF-alpha (tumor necrosis factor alpha) , IL-6 (interleukin 6) and MCP-1 (monocyte chemoattractant protein-1), with a decreased production of adiponectin and a raised production of leptin. The adipocytes, such as leukocytes and endothelial cells, express and secrete inflammatory mediators, contributing of the atherogenic process and the evolution of cardiovascular disease. PPARs are also important in control of several biological processes mainly related to lipid metabolism and inflammation, contributing, to the development of cardiovascular diseases. Recent studies have suggested that oxygen deprivation is closely associated with certain pathologies such as Metabolic Syndrome, Obesity, Type 2 Diabetes and cardiovascular disease. Although the use of nicotinic acid for the treatment of dyslipidemia is already known. Recent studies have demonstrated the molecular and cellular mechanisms of nicotinic acid action on the regulation in the metabolism of lipids and lipoproteins. The nicotinic acid has effects on reducing of triglycerides stocks in adipose tissue, and it inhibits the diacylglycerol acetyltransferase released by hepatocytes. Nicotinic acid also acts on reduced synthesis of triglycerides, increasing the degradation of apolipoprotein B, and consequently decreasing the secretion of VLDL (Very Low Density Lipoprotein) and LDL (Low Density Lipoprotein). Based on anti-inflammatory properties and the reduction of cardiovascular disease development by treatment with nicotinic acid and the importance of adipocytes in the evolution of atherogenesis, this study aims to investigate the impact of the pleiotropic properties of nicotinic acid in adiponectin, leptin and PAI-1 secretion and in PPAR-gamma and PPAR-alpha expression in adipocytes culture under oxigen deprivation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAKAMICHI, RENATA; MIRANDA, ERIKA PRATES; DE VERGUEIRO LOBO, SYLVIA MADEIRA; TRISTAO, VIVIAN REGINA; DALBONI, MARIA APARECIDA; REDUBLO QUINTO, BEATA MARIE; BATISTA, MARCELO COSTA. Action of nicotinic acid on the reversion of hypoxic-inflammatory link on 3T3-L1 adipocytes. LIPIDS IN HEALTH AND DISEASE, v. 15, MAY 10 2016. (11/07248-2)