Evaluation of curcumin and cisplatin-induced DNA damage in PC12 cells by the alkaline comet assay

Autor(es)

Mendonca, Leonardo Meneghin ^[1] ; dos Santos, Graciela Cristina ^[2] ; dos Santos, Raquel Alves ^[3] ; Takahashi, Catarina Satie ^{[4, 3]} ; Pires Bianchi, Maria de Lourdes ^[1] ; Greggi Antunes, Lusania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Alimentos & Nutr, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN & EXPERIMENTAL TOXICOLOGY; v. 29, n. 8, p. 635-643, AUG 2010.
• Citações Web of Science: 9

Resumo

A very appropriate method for antigenotoxicity evaluation of antioxidants is the comet assay, since this analytical method detects initial DNA lesions that are still subject to repair; in other words, lesions that are very associated to damages resulting from the generation and subsequent action of reactive species. However, a solid evaluation should be developed in order to avoid inexact interpretations. In our study, besides the association of curcumin with cisplatin, curcumin and cisplatin agents were also tested separately. Classical genotoxic compounds, when tested by the comet assay, present an increase in the nucleoid tail; however, the cisplatin treatment has resulted in a decrease of DNA migration. This was an expected effect, as the cross-links between cisplatin and DNA decrease the DNA electrophoretic mobility. A similar effect was observed with the curcumin treatment, which decreased the nucleoid tail. Such effect was not expected and reinforced the necessity of including in the study, separate treatment groups with potentially antigenotoxic substances. The comet assay results have been analyzed using specific software for image analysis, as well as the classical visual analysis, and we have observed that the effect of decrease in DNA electrophoretic mobility was more easily observed when the data were analyzed by the software. (AU)

• Processo FAPESP: 08/53947-7 - Neurotoxicidade induzida pelo quimioterápico cisplatina: possíveis efeitos citoprotetores dos antioxidantes da dieta curcumina e coenzima Q10
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular