Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay

Autor(es)

de Souza, Sibele Pinheiro ^{[1, 2]} ; Asano, Karen Miyuki ^{[1, 2]} ; Sandri, Thaisa Lucas ^{[1, 2]} ; de Barros, Iracema Nunes ^{[1, 2]} ; Richtzenhain, Leonardo Jose ^{[1, 2]} ; Brandao, Paulo Eduardo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet Zootecnia, BR-05508270 Sao Paulo, SP - Brazil

^[2] Grp Pesquisa Coronavirus, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 41, n. 3, p. 810-812, JUL-OCT 2010.
• Citações Web of Science: 0

Resumo

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation. (AU)

• Processo FAPESP: 07/59108-4 - Disenteria de inverno em bovinos leiteiros adultos no estado de São Paulo: detecção e genealogia de amostras entéricas de coronavírus bovino
• Beneficiário: Paulo Eduardo Brandão
• Modalidade de apoio: Auxílio à Pesquisa - Regular