Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA

Autor(es)

Goldfeder, Mauricio B. ; Oliveira, Carla C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem, Inst Chem, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS Journal; v. 277, n. 13, p. 2838-2852, JUL 2010.
• Citações Web of Science: 17

Resumo

In eukaryotes, pre-rRNA processing depends on a large number of nonribosomal trans-acting factors that form intriguingly organized complexes. Two intermediate complexes, pre-40S and pre-60S, are formed at the early stages of 35S pre-rRNA processing and give rise to the mature ribosome subunits. Each of these complexes contains specific pre-rRNAs, some ribosomal proteins and processing factors. The novel yeast protein Utp25p has previously been identified in the nucleolus, an indication that this protein could be involved in ribosome biogenesis. Here we show that Utp25p interacts with the SSU processome proteins Sas10p and Mpp10p, and affects 18S rRNA maturation. Depletion of Utp25p leads to accumulation of the pre-rRNA 35S and the aberrant rRNA 23S, and to a severe reduction in 40S ribosomal subunit levels. Our results indicate that Utp25p is a novel SSU processome subunit involved in pre-40S maturation. (AU)

• Processo FAPESP: 07/57096-9 - Caracterização funcional de novas proteínas de Saccharomyces cerevisiae envolvidas em processamento de RNA: papel de Nop8p e kr071p na maturação do pre-60s
• Beneficiário: Carla Columbano de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular