Pathology and Tissue Distribution of Turkey Coronavirus in Experimentally Infected Chicks and Turkey Poults

Autor(es)

Gomes, D. E. ^[1] ; Hirata, K. Y. ^[1] ; Saheki, K. ^[1] ; Rosa, A. C. G. ^[1] ; Luvizotto, M. C. R. ^[2] ; Cardoso, T. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, UNESP, Sch Vet, Lab Anim Virol, DAPSA, BR-16050680 Aracatuba, SP - Brazil

^[2] Univ Sao Paulo State, UNESP, Sch Vet, Lab Vet Pathol, DCCRA, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Comparative Pathology; v. 143, n. 1, p. 8-13, JUL 2010.
• Citações Web of Science: 4

Resumo

Twenty 1-day-old specific pathogen free chicks and 20 1-day-old commercially derived turkey poults were inoculated with a Brazilian strain of turkey coronavirus (TCoV) to study the pathogenicity and virus distribution up to 14 days post-moculanon by histopathology, immunohistochemistry, reverse transcriptase polymerase chain reaction and sequencing. At 2-14 dpi, TCoV antigens were detected in the paranasal sinus and lachrymal accessory gland (Harderian gland) of infected chicks and in the ileum, ileocaecal junction and caecum of infected poults Lymphocytic inflammation was present in these tissues TCoV was re-isolated from pooled tissue suspensions of nasal concha, Harderian gland and paranasal sinus from chicks, as well as from the ileum, ileocaecal junction and caecum of poults, after three consecutive passages in 28-day-old embryonated turkey eggs Viral RNA corresponding to the spike gene region (1178-2073 genome position) was amplified from the upper respiratory tract of chickens and from the intestinal tract of poults and phylogenetic analysis confirmed the identity as TCoV This is the first description of TCoV antigens and mRNA in upper respiratory tissues in experimentally infected chickens (C) 2010 Elsevier Ltd All rights reserved (AU)

• Processo FAPESP: 08/50380-6 - Análise filogenética e alterações patológicas do Coronavirus de perus isolado no Brasil de casos clínicos de Poult Enteritis Mortality Syndrome após inoculação experimental de perus jovens e ovos embrionados SPF de galinhas
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/56041-6 - Carcterizacao molecular do Turkey coronavírus no Brasil: análise da variabilidade da glicoproteína S (gene 3)
• Beneficiário: Deriane Elias Gomes
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/52994-3 - Susceptibilidade do embriao de avestruz (struchio camelus) a infeccao experimental com o virus vacinal (lukert) e a estirpe virulenta do virus da doenca infecciosa da bursa (vvvdib).
• Beneficiário: Analy Ramos Mendes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica