Th1/Th2 cytokines' expression and production by propolis-treated mice

Autor(es)

Orsatti, Claudio Lera ^[1] ; Missima, Fabiane ^[1] ; Pagliarone, Ana Carolina ^[1] ; Sforcin, Jose Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Biosci Inst, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 129, n. 3, p. 314-318, JUN 16 2010.
• Citações Web of Science: 32

Resumo

Aim of the study: Propolis is a natural product extensively used in food and beverages to improve health and to prevent diseases, showing immunomodulatory properties. The goal of this work was to evaluate the effect of propolis administration over a short-term to mice on Th1 (IL-2 and IFN-gamma) and Th2 (IL-4 and IL-10) cytokines' expression and production. Materials and methods: Propolis was administered for 3 days to mice by gavage, spleens were removed and RNA was extracted to assess cytokines' expression by real-time PCR. Supernatants of spleen cell cultures were used for cytokines determination by ELISA. Results: Propolis administration to mice did not affect IL-2, IL-4 and IL-10 expression and production, while IFN-gamma production was inhibited in the splenocyte cultures stimulated or not by Con A. Conclusions: Since IFN-gamma is a pro-inflammatory cytokine, our data suggest that propolis administration over a short-term to mice may be associated with anti-inflammatory effects in vivo, and further assays could check propolis efficiency in inflammatory diseases. (C) 2010 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/58830-5 - Efeito da própolis in vivo sobre a expressão de receptores semelhantes a Toll (TLR4 etlr2) e produção de citocinas por camundongos Balb/c
• Beneficiário: Cláudio Lera Orsatti
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/02692-6 - Expressão de TLR-2 e TLR-4 e produção de citocinas por esplenócitos de camundongos submetidos a estresse agudo
• Beneficiário: José Maurício Sforcin
• Modalidade de apoio: Auxílio à Pesquisa - Regular