Diversity and identification of methanogenic archaea and sulphate-reducing bacteria in sediments from a pristine tropical mangrove

Autor(es)

Taketani, Rodrigo Gouvea ^[1] ; Yoshiura, Caio Augusto ^[1] ; Franco Dias, Armando Cavalcante ^{[1, 2]} ; Andreote, Fernando Dini ^[2] ; Tsai, Siu Mui ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, SP - Brazil

^[2] EMBRAPA, Lab Environm Microbiol, Jaguariuna, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 97, n. 4, p. 401-411, MAY 2010.
• Citações Web of Science: 41

Resumo

Mangrove sediments are anaerobic ecosystems rich in organic matter. This environment is optimal for anaerobic microorganisms, such as sulphate-reducing bacteria and methanogenic archaea, which are responsible for nutrient cycling. In this study, the diversity of these two functional guilds was evaluated in a pristine mangrove forest using denaturing gradient gel electrophoresis (DGGE) and clone library sequencing in a 50 cm vertical profile sampled every 5.0 cm. DGGE profiles indicated that both groups presented higher richness in shallow samples (0-30 cm) with a steep decrease in richness beyond that depth. According to redundancy analysis, this alteration significantly correlated with a decrease in the amount of organic matter. Clone library sequencing indicated that depth had a strong effect on the selection of dissimilatory sulphate reductase (dsrB) operational taxonomic units (OTUs), as indicated by the small number of shared OTUs found in shallow (0.0 cm) and deep (40.0 cm) libraries. On the other hand, methyl coenzyme-M reductase (mcrA) libraries indicated that most of the OTUs found in the shallow library were present in the deep library. These results show that these two guilds co-exist in these mangrove sediments and indicate important roles for these organisms in nutrient cycling within this ecosystem. (AU)

• Processo FAPESP: 06/06700-0 - Diversidade microbial em terra preta antropongênica da Amazônia Central e Oriental: detecção de Archaea metanogênicas, seu papel funcional e sua contribuição na estrutura das comunidades bacterianas
• Beneficiário: Tsai Siu Mui
• Linha de fomento: Auxílio à Pesquisa - Regular