Neither Molecular Diversity of the Envelope, Immunosuppression Status, Nor Proviral Load Causes Indeterminate HTLV Western Blot Profiles in Samples From Human T-Cell Lymphotropic Virus Type 2 (HTLV-2)-Infected Individuals

Autor(es)

Olah, Ingrid ^[1] ; Fukumori, Ligia M. I. ^[1] ; Smid, Jerusa ^[2] ; Penalva de Oliveira, Augusto Cesar ^[2] ; Duarte, Alberto J. S. ^[1] ; Casseb, Jorge ^{[3, 2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Lab Dermatol & Immunol, Sao Paulo - Brazil

^[2] Inst Infect Dis Emilio Ribas, HTLV Outpatient Clin, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 82, n. 5, p. 837-842, MAY 2010.
• Citações Web of Science: 7

Resumo

Although human T-cell lymphotropic virus type 2 (HTLV-2) is considered of low pathogenicity, serological diagnosis is important for counseling and monitoring. The confirmatory tests most used are Western blot (WB) and PCR. However, in high-risk populations, about 50% of the indeterminate WB were HTLV-2 positives by PCR. The insensitivity of the WB might be due to the use of recombinant proteins of strains that do not circulate in our country. Another possibility may be a high level of immunosuppression, which could lead to low production of virus, resulting in low stimulation of antibody. We found one mutation, proline to serine in the envelope region in the position 184, presented at least 1/3 of the samples, independent the indeterminate WB profile. In conclusion, we found no correlation of immune state, HTLV-2 proviral load, or env diversity in the K55 region and WB indeterminate results. We believe that the only WB kit available in the market is probably more accurate to detect HTLV-1 antibodies, and some improvement for HTLV-2 detection should be done in the future, especially among high-risk population. J. Med. Virol. 82:837-842,2010. (C) 2010 Wiley-Liss, Inc. (AU)

• Processo FAPESP: 07/52298-2 - Diversidade molecular do envelope e carga proviral do virus linfotropico de celulas t humanas tipo 2 (htlv-2) em amostras indeterminadas pelo teste wetern-blot
• Beneficiário: Ingrid Olah Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/56427-4 - Diversidade molecular do envelope e carga proviral do Vírus Linfotrópico de Células T Humanas tipo 2 (HTLV-2) em amostras indeterminadas pelo teste Western-Blot
• Beneficiário: Jorge Simao do Rosario Casseb
• Modalidade de apoio: Auxílio à Pesquisa - Regular