Single embryo and oocyte lipid fingerprinting by mass spectrometry

Autor(es):Mostrar menos -

Ferreira, Christina R. ^[1] ; Saraiva, Sergio A. ^[1] ; Catharino, Rodrigo R. ^[2] ; Garcia, Jerusa S. ^[1] ; Gozzo, Fabio C. ^[3] ; Sanvido, Gustavo B. ^[1] ; Santos, Luiz Fernando A. ^[3] ; Lo Turco, Edson G. ^[4] ; Pontes, Jose Henrique F. ^[5] ; Basso, Andrea C. ^[5] ; Bertolla, Ricardo P. ^[4] ; Sartori, Roberto ^[6] ; Guardieiro, Monique M. ^[6] ; Perecin, Felipe ^[7] ; Meirelles, Flvio V. ^[7] ; Sangalli, Juliano R. ^[7] ; Eberlin, Marcos N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Clin Pathol, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Ctr Struct Mol Biol, Campinas, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Surg, Sao Paulo - Brazil

^[5] Vitro Brasil Ltda, Mogi Mirim, SP - Brazil

^[6] Univ Sao Paulo, Dept Anim Sci, Piracicaba, SP - Brazil

^[7] Univ Sao Paulo, Fac Anim Sci & Food Engn, Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Lipid Research; v. 51, n. 5, p. 1218-1227, MAY 2010.
• Citações Web of Science: 74

Resumo

Methods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.-Ferreira, C. R., S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F. A. Santos, E. G. Lo Turco, J. H. F. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M. Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, and M. N. Eberlin. Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 2010. 51: 1218-1227. (AU)

• Processo FAPESP: 08/10756-7 - Aplicação da técnica de espectrometria de massas para caracterização do lipidoma em oócitos e embriões bovinos
• Beneficiário: Christina Ramires Ferreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado