Quantification of chlorpheniramine maleate enantiomers by ultraviolet spectroscopy and chemometric methods

Autor(es)

Valderrama, P. ^[1] ; Romero, A. L. ^[1] ; Imamura, P. M. ^[1] ; Magalhaes, I. R. S. ^[2] ; Bonato, P. S. ^[2] ; Poppi, R. J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 397, n. 1, p. 181-188, MAY 2010.
• Citações Web of Science: 10

Resumo

Chlorpheniramine maleate (CLOR) enantiomers were quantified by ultraviolet spectroscopy and partial least squares regression. The CLOR enantiomers were prepared as inclusion complexes with beta-cyclodextrin and 1-butanol with mole fractions in the range from 50 to 100%. For the multivariate calibration the outliers were detected and excluded and variable selection was performed by interval partial least squares and a genetic algorithm. Figures of merit showed results for accuracy of 3.63 and 2.83% (S)-CLOR for root mean square errors of calibration and prediction, respectively. The ellipse confidence region included the point for the intercept and the slope of 1 and 0, respectively. Precision and analytical sensitivity were 0.57 and 0.50% (S)-CLOR, respectively. The sensitivity, selectivity, adjustment, and signal-to-noise ratio were also determined. The model was validated by a paired t test with the results obtained by high-performance liquid chromatography proposed by the European pharmacopoeia and circular dichroism spectroscopy. The results showed there was no significant difference between the methods at the 95% confidence level, indicating that the proposed method can be used as an alternative to standard procedures for chiral analysis. (AU)

• Processo FAPESP: 05/56188-1 - Avaliacao de figuras de merito em calibracao multivariada na determinacao da composicao enantiomerica de moleculas de interesse farmaceutico atraves de espectroscopia por complexacao com...
• Beneficiário: Patrícia Valderrama
• Modalidade de apoio: Bolsas no Brasil - Doutorado