Characterization of cytotoxic immune response in skin and mucosal lesions of paracoccidioidomycosis

Autor(es)

Pagliari, Carla ^{[1, 2]} ; Pereira, Naiura Vieira ^{[1, 2]} ; Kanashiro, Luciane ^[2] ; Stegun, Felipe Weisshaupt ^[2] ; Croda, Julio ^[3] ; Seixas Duarte, Maria Irma ^[2] ; Sotto, Mirian Nacagami ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Sci, Lab Dermatopathol, Dept Dermatol, BR-01246903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Sci, Lab Discipline Pathol Transmissible Dis, Dept Pathol, BR-01246903 Sao Paulo - Brazil

^[3] Univ Grande Dourados, Fac Hlth Sci, Dourados - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CUTANEOUS PATHOLOGY; v. 37, n. 5, p. 565-570, MAY 2010.
• Citações Web of Science: 4

Resumo

Background: CD8+ T cells and natural killer (NK) cells are involved in the immune response against some pathogens. For this purpose, we investigated the in situ paracoccidioidomycosis (PCM) immune response addressing the participation of NK cells, CD8+ T cells, perforin and granzyme B expression. Methods: Sixty biopsies of PCM skin and mucosa were classified according to the presence of compact granulomas (G1), poorly organized granulomas (G2) and both kinds in the same lesion (G3). CD8+ T cells, NK cells, perforin and granzyme B were showed by immunohistochemistry. Results: CD8+ T cells were increased over NK cells in cutaneous G1 and G2 lesions. There was no difference regarding such cells in G3 lesions, although they were abundant in such lesions. In mucosa, CD8+ T cells were increased in number over NK cells in all groups. Granzyme B in skin increased in G2 and G3. The number of granzyme did not differ in mucosal lesions in the three groups. Conclusions: CD8+ T cells and NK cells play a role in PCM cutaneous and mucosal lesions. The predominance of CD8+ T cells over NK cells may represent an effective response against the fungi. Moreover, the high number of granzyme B expressing cells corroborates this possibility. (AU)

• Processo FAPESP: 07/00916-4 - Resposta imune celular in situ na paracoccidioidomicose: estudo de citocinas, mediadores citotoxicos e apoptose em lesoes cutaneas e de mucosa oral.
• Beneficiário: Carla Pagliari
• Modalidade de apoio: Auxílio à Pesquisa - Regular