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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Major Histocompatability Complex Class I Expression and Glial Reaction Influence Spinal Motoneuron Synaptic Plasticity During the Course of Experimental Autoimmune Encephalomyelitis

Texto completo
Autor(es):
Freria, C. M. [1] ; Zanon, R. G. [1] ; Santos, L. M. B. [2] ; Oliveira, A. L. R. [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Lab Nerve Regenerat, Dept Anat, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Microbiol & Immunol, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF COMPARATIVE NEUROLOGY; v. 518, n. 7, p. 990-1007, APR 1 2010.
Citações Web of Science: 22
Resumo

Recent studies have shown that major histocompatibility complex class I (MHC I) expression directly influences the stability of nerve terminals. Also, the acute phase of experimental autoimmune encephalomyelitis (EAE) has shown a significant impact on inputs within the spinal cord. Therefore, the present work investigated the synaptic covering of motoneurons during the induction phase of disease and progressive remissions of EAE. EAE was induced in C57BL/6J mice, which were divided into four groups: normal, peak disease, first remission, and second remission. The animals were killed and their lumbar spinal cords processed for in situ hybridization (IH), immunohistochemistry, and transmission electron microscopy (TEM). The results indicated an increase in glial reaction during the peak disease. During this period, the TEM analysis showed a reduction in the synaptic covering of the motoneurons, corresponding to a reduction in synaptophysin immunolabeling and an increase in the MHC I expression. The IH analysis reinforced the immunolabeling results, revealing an increased expression of MHC I mRNA by motoneurons and nonneuronal cells during the peak disease and first remission. The results observed in both remission groups indicated a return of the terminals to make contact with the motoneuron surface. The ratio between excitatory and inhibitory inputs increased, indicating the potential for development of an excitotoxic process. In conclusion, the results presented here indicate that MHC I up-regulation during the course of EAE correlates with the periods of synaptic plasticity induced by the infiltration of autoreactive immune cells and that synaptic plasticity decreases after recurrent peaks of inflammation. J. Comp. Neurol. 518:990-1007, 2010. (C) 2009 Wiley-Liss, Inc. (AU)

Processo FAPESP: 07/03645-1 - Análise da plasticidade sináptica em motoneurônios medulares de animais submetidos a encefalomielite autoimune experimental
Beneficiário:Alexandre Leite Rodrigues de Oliveira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/16879-6 - MHC i expression and glial reaction influence spinal motoneuron synaptic plasticity during the course of experimental autoimmune encephalomyelitis
Beneficiário:Alexandre Leite Rodrigues de Oliveira
Linha de fomento: Auxílio à Pesquisa - Publicações científicas - Artigo
Processo FAPESP: 06/05055-4 - Análise de plasticidade sináptica em motoneurônios medulares de animais submetidos a encefalomielite autoimune experimental
Beneficiário:Camila Marques de Freria
Linha de fomento: Bolsas no Brasil - Mestrado