The 434(G > C) polymorphism in the eosinophil cationic protein gene and its association with tissue eosinophilia in oral squamous cell carcinomas

Autor(es)

Pereira, Michele C. ^[1] ; Oliveira, Denise T. ^[1] ; Olivieri, Eloisa H. R. ^[2] ; Rogatto, Silvia R. ^[3] ; Carvalho, Andre L. ^[4] ; Landman, Gilles ^[5] ; Kowalski, Luiz P. ^[6]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Stomatol, Area Pathol, Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Dept Genet, Inst Biosci, Sao Paulo - Brazil

^[3] Sao Paulo State Univ, Dept Urol, Fac Med, Sao Paulo - Brazil

^[4] Canc Hosp Barretos, Dept Head & Neck Surg, Fundacao Pio 12, Sao Paulo - Brazil

^[5] Canc Hosp AC Camargo, Dept Pathol, Sao Paulo - Brazil

^[6] Canc Hosp AC Camargo, Dept Head & Neck Surg & Otorhinolaryngol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 39, n. 1, p. 56-62, JAN 2010.
• Citações Web of Science: 4

Resumo

Objective: The aim of this study was to investigate the prevalence of the Eosinophil cationic protein (ECP)-gene polymorphism 434(G > C) in oral squamous cell carcinoma (OSCC) patients and its association with tumor-associated tissue eosinophilia (TATE), demographic, clinical, and microscopic variables. Methods: The ECP genotypes of 165 healthy individuals and 157 OSCC patients were detected by PCR-RFLP analysis after cleavage of the amplified DNA sequence with enzyme PstI. TATE was obtained by morphometric analysis. Chi-square test or Fisher's exact test was used to analyze the association of ECP-gene polymorphism 434(G > C) with TATE, demographic, clinical, and microscopic variables in OSCC patients. Disease-free survival and overall survival were calculated by the Kaplan-Meier product-limit actuarial method and the comparison of the survival curves were performed using log rank test. Results: Most of healthy individuals (53.33%) and OSCC patients (57.97%) were heterozygous for the ECP 434(G > C) polymorphism. Based on numerical differences, our results showed that OSCC patients with intense TATE and at least one C allele had a higher frequency of bilateral neck dissection, local recurrence, vascular embolization, involved resection margins, and postoperative radiotherapy. No statistically significant differences on survival rates were found in OSCC patients presenting different ECP 434(G > C) genotypes. Conclusions: These results suggest a tendency towards a poor clinical outcome in OSCC patients with intense TATE and 434GC/CC genotypes, probably due to an ECP genetic variant with altered cytotoxic activity. (AU)

• Processo FAPESP: 06/03830-0 - Associação do polimorfismo do gene da proteína catiônica eosinofílica com a eosinofilia tecidual associada aos tumores em carcinomas espinocelulares de boca
• Beneficiário: Denise Tostes Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular