Azure dyes as new photosensitizer prototypes to application in photodynamic therapy against Candida spp.

Autor(es)

Moreira, Leonardo M. ^[1] ; Lyon, Juliana P. ^[2] ; Tursi, Suellen M. S. ^[3] ; Trajano, Isis ^[3] ; Felipe, Monalisa P. ^[3] ; Costa, Maricilia S. ^[3] ; Rodrigues, Maira R. ^[4] ; Codognoto, Lucia ^[5] ; de Oliveira, Hueder P. M. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Joao del Rei, Dept Engn Biossistemas, Sao Joao Del Rei, MG - Brazil

^[2] Univ Fed Sao Joao del Rei, Dept Ciencias Nat, Sao Joao Del Rei, MG - Brazil

^[3] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, SP - Brazil

^[4] Polo Univ Rio das Ostras, Univ Fed Fluminense, Dept Ciencia Tecnol, Rio Das Ostras, RJ - Brazil

^[5] Univ Camilo Castelo Branco, BR-12247004 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPECTROSCOPY-AN INTERNATIONAL JOURNAL; v. 24, n. 6, p. 621-628, 2010.
• Citações Web of Science: 6

Resumo

Infections caused by Candida albicans are of increasing concern, especially considering immunodepressed patients. The toxicity of most antifungal agents, the great number of cases with recidives, as well as the emergence of resistant samples has provoked the evaluation of new forms of therapy. In this context, the photodynamic therapy (PDT) presents auspicious antimicrobial properties, stimulating the development of trials employing several kinds of photosensitizers. In the present work, the application of different kind of Azure dyes as photosensitizer in PDT against C. albicans was evaluated through instrumental measurements of electronic spectroscopy. In fact, the values of optical density were a precise indicator of the growth inhibition of the microorganisms. Indeed, Azures are phenothiazinium derivatives that constitute a very relevant class of compounds with several biomedical applications, such as photoantimicrobial therapy against local bacterial infection, tuberculosis, trypanosomiasis, malaria, Rickettsia, yeasts, viral infection n and cancer. Azure A, Azure B, Azure A thiocyanate, Azure B BF(4), Azure A eosinate are the dyes tested against C. albicans. The results denoted completely distinct behaviors to the different types of Azure compound evaluated in this work. In fact, Azure A and Azure A eosinate presented significant results when irradiated with 56 J/cm(2), since the growth inhibition of C. albicans reached approximately 60%. This Azure compounds have significant potential to be employed as photosensitizer (PS) in PDT, especially in cases of mucocutaneous candidosis. The spectroscopic evaluation was very effective to the detection of slight alterations in the growth of the microorganisms, denoting that this kind of analysis is an excellent alternative to determine growth inhibition of Candida albicans. The experimental data are discussed in details in agreement with recent results from literature. (AU)

• Processo FAPESP: 02/00272-6 - Estudo e caracterização de polissacarídeos hidrofobicamente modificados
• Beneficiário: Maira Regina Rodrigues Magini
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 06/56701-3 - Estudo de processos de agregacao em moleculas com potencial aplicacao em terapia fotodinamica.
• Beneficiário: Hueder Paulo Moisés de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/50588-6 - Eletrodos de ouro modificados por monocamadas auto-organizadas aplicados à determinação de pesticidas
• Beneficiário: Lucia Codognoto de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/07162-5 - Avaliação da atividade de superfactantes sobre fatores de virulência de levedura do gênero Candida
• Beneficiário: Juliana Pereira Lyon
• Modalidade de apoio: Auxílio à Pesquisa - Regular