Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring

Autor(es):Mostrar menos -

Chiaratti, Marcos Roberto ^[1] ; Ferreira, Christina Ramires ^{[2, 1]} ; Meirelles, Flavio Vieira ^[1] ; Meo, Simone Cristina ^{[3, 2]} ; Perecin, Felipe ^[1] ; Smith, Lawrence Charles ^[4] ; Ferraz, Marcio Leao ^[5] ; de Sa Filho, Manoel Francisco ^[6] ; Gimenes, Lindsay Unno ^[6] ; Baruselli, Pietro Sampaio ^[6] ; Gasparrini, Bianca ^[7] ; Garcia, Joaquim Mansano

Afiliação do(s) autor(es)

^[1] USP, Dept Ciencias Basicas, FZEA, Pirassununga, SP - Brazil

^[2] UNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP - Brazil

^[3] Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil

^[4] Univ Montreal, CRRA, St Hyacinthe, PQ - Canada

^[5] Vida Reprodut Consultoria, Cravinhos, SP - Brazil

^[6] Univ Sao Paulo, Dept Anim Reprod, Sao Paulo - Brazil

^[7] Univ Naples Federico 2, DISCIZIA, Naples - Italy

• Tipo de documento: Artigo Científico
• Fonte: CELLULAR REPROGRAMMING; v. 12, n. 3, p. 231-236, 2010.
• Citações Web of Science: 7

Resumo

Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were not compromised by XOT in comparison to both in vitro fertilized embryos and embryos produced by transfer of bovine ooplasm into bovine zygotes. Moreover, offspring were born after transfer of XOT embryos to recipient cows. Buffalo mtDNA introduced in zygotes was still present at the blastocyst stage (8.3 vs. 9.3%, p = 0.11), indicating unaltered heteroplasmy during early development. Nonetheless, no vestige of buffalo mtDNA was found in offspring, indicating a drift to homoplasmy during later stages of development. In conclusion, we show that the buffalo mtDNA introduced by XOT into a bovine zygote do not compromise embryo development. On the other hand, buffalo mtDNA was not inherited by offspring indicating a possible failure in the process of interspecies mtDNA replication. (AU)

• Processo FAPESP: 04/01841-0 - Controle da herança mitocondrial em embriões bovinos micromanipulados
• Beneficiário: Joaquim Mansano Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/05054-7 - Controle da herança mitocondrial em embriões bovinos micromanipulados
• Beneficiário: Christina Ramires Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/59074-0 - Efeito da quantidade de mitocôndrias e de DNA mitocondrial sobre o desenvolvimento embrionário bovino: dois modelos originais
• Beneficiário: Flávio Vieira Meirelles
• Modalidade de apoio: Auxílio à Pesquisa - Regular