EXPRESSION OF GENES RELATED TO MYOSTATIN SIGNALING DURING RAT SKELETAL MUSCLE LONGITUDINAL GROWTH

Autor(es)

Aoki, Marcelo S. ^{[1, 2]} ; Soares, Antonio G. ^[2] ; Miyabara, Elen H. ^[3] ; Baptista, Igor L. ^[2] ; Moriscot, Anselmo S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-03828000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-03828000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MUSCLE & NERVE; v. 40, n. 6, p. 992-999, DEC 2009.
• Citações Web of Science: 19

Resumo

In this study we investigated the gene expression of proteins related to myostatin (MSTN) signaling during skeletal muscle longitudinal growth. To promote muscle growth, Wistar male rats were submitted to a stretching protocol for different durations (12, 24, 48, and 96 hours). Following this protocol, soleus weight and length and sarcomere number were determined. In addition, expression levels of the genes that encode MSTN, follistatin isoforms 288 and 315 (FLST288 and FLST315), follistatin-like 3 protein (FLST-L3), growth and differentiation factor-associated protein-1 (GASP-1), activin IIB receptor (ActIIB), and SMAD-7 were determined by real-time polymerase chain reaction. Prolonged stretching increased soleus weight, length, and sarcomere number. In addition, MSTN gene expression was increased at 12-24 hours, followed by a decrease at 96 hours when compared with baseline values. FLST isoforms, FLST-L3, and GASP-1 mRNA levels increased significantly over all time-points. ActIIB gene expression decreased quickly at 12-24 hours. SMAD-7 mRNA levels showed a late increase at 48 hours, which peaked at 96 hours. The gene expression pattern of inhibitory proteins related to MSTN signaling suggests a strong downregulation of this pathway in response to prolonged stretching. Muscle Nerve 40: 992-999, 2009 (AU)

• Processo FAPESP: 06/52204-5 - Regulacao da expressao do gene da miostatina (gdf-8) durante o crescimento longitudinal do musculo esqueletico.
• Beneficiário: Marcelo Saldanha Aoki
• Modalidade de apoio: Auxílio à Pesquisa - Regular