Distribution and hydrolytic enzyme characteristics of Candida albicans strains isolated from diabetic patients and their non-diabetic consorts

Autor(es)

Boriollo, M. F. G. ^[1] ; Bassi, R. C. ^[2] ; dos Santos Nascimento, C. M. G. ^[3] ; Feliciano, L. M. ^[3] ; Francisco, S. B. ^[3] ; Barros, L. M. ^[4] ; Spolidorio, L. C. ^[5] ; Palomari Spolidorio, D. M. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Alfenas UNIFENAS, Fac Ciencias Med, Lab Genet & Biol Mol, Sch Med, BR-37130000 Alfenas, MG - Brazil

^[2] Univ Alfenas, Biomed Sch, Alfenas, MG - Brazil

^[3] Einstein Integrated Fac, Limeira Biomed Sch, Limeira, SP - Brazil

^[4] Univ Alfenas, Sch Dent, Alfenas, MG - Brazil

^[5] State Univ Sao Paulo, Araraquara Dent Sch, Oral Pathol & Physiol Dept, Oral Microbiol & Immunol Lab, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oral Microbiology and Immunology; v. 24, n. 6, p. 437-450, DEC 2009.
• Citações Web of Science: 15

Resumo

Introduction: The aim of this study was to investigate the oral colonization profile of Candida albicans strains isolated from diabetic patients and their non-diabetic consorts. In addition hydrolytic enzyme activity of these isolates was analysed. Methods: The genetic diversity of C. albicans oral isolates from 52 couples was established using isoenzyme marker and cluster analysis. Hydrolytic enzyme characteristics, namely secreted aspartyl proteinases (SAPs) and phospholipases (PLs) were also analysed. Results: Simultaneous colonization by C. albicans was observed in the consorts of 12 couples (23.1%). Patterns of monoclonal and polyclonal oral colonization by C. albicans strains were identified and the coexistence of identical or highly related strains was observed in both members of eight couples. The genetic diversity observed in the total yeast population revealed four large, genetically distinct groups (A to D) and the coexistence of strains in couples or consorts conjugally unrelated. SAP and PL activity was observed in the majority of C. albicans isolates without any association to particular strain, strain clusters (highly related isolates), or clinical characteristics of the consorts (diabetic, non-diabetic, and gender). Conclusion: Possible sources of transmission and oral propagation of groups (clusters) of strains of C. albicans can occur between diabetic and non-diabetic consorts. A conjugal genotypic identity exists in most C. albicans-positive couples, that is, both consorts share identical or highly related strains; however, this identity is not couple-specific as seen by the coexistence of clusters in couples and unrelated consorts. (AU)

• Processo FAPESP: 05/50673-5 - Eletroforese de enzimas multilocos (MLEE) e análises de agrupamento de isolados bucais de Candida albicans provenientes de pacientes com diabetes mellitus e membros familiares saudáveis
• Beneficiário: Denise Madalena Palomari Spolidorio
• Modalidade de apoio: Auxílio à Pesquisa - Regular