Microsatellites from rubber tree (Hevea brasiliensis) for genetic diversity analysis and cross-amplification in six Hevea wild species

Autor(es)

Souza, L. M. ^[1] ; Mantello, C. C. ^[1] ; Santos, M. O. ^[1] ; de Souza Goncalves, P. ^{[2, 3]} ; Souza, Anete Pereira ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil

^[2] IAC, BR-13012970 Campinas, SP - Brazil

^[3] Empresa Brasileira Pesquisa Agropecuaria Embrapa, Brasilia, DF - Brazil

^[4] Univ Estadual Campinas, Dept Biol Vegetal, Inst Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS RESOURCES; v. 1, n. 1, p. 75-79, DEC 2009.
• Citações Web of Science: 21

Resumo

Hevea brasiliensis is native to the Amazonian rain forest and an important source of natural rubber. Twenty seven polymorphic microsatellite loci were isolated and characterized from a GA-CA enriched genomic library of H. brasiliensis. The number of alleles ranged from 2 to 20. The observed and expected heterozygosity ranged from 0.13 to 0.88 and from 0.00 to 0.89, respectively. Cross-species amplification of the markers developed for H. brasiliensis was successful in the wild Hevea species H. guianensis, H. rigidifolia, H. nitida, H. pauciflora, H. benthamiana and H. camargoana. The data indicated a high degree of sequence homology in the microsatellite flanking regions of these species. The developed SSR loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow among Hevea species. (AU)

• Processo FAPESP: 07/50392-1 - Mapa genético-molecular para Hevea brasiliensis e mapeamento de QTLs para características de importância econômica
• Beneficiário: Livia Moura de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/59804-0 - Desenvolvimento de marcadores moleculares do tipo microssatélite em Hevea brasiliensis
• Beneficiário: Camila Campos Mantello
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica