Differentiation of field isolates and vaccine strains of infectious laryngotracheitis virus by DNA sequencing

Autor(es)

Chacon, Jorge Luis ; Piantino Ferreira, Antonio J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Coll Vet Med, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Vaccine; v. 27, n. 48, p. 6731-6738, NOV 12 2009.
• Citações Web of Science: 29

Resumo

Two different regions of the infected cell protein 4 (ICP4) gene of infectious laryngotracheitis virus (ILTV) were amplified and sequenced for characterization of field isolates and tissue culture-origin (TCO) and chicken embryo-origin (CEO) vaccine strains. Phylogenetic analysis of the two regions showed differences in nucleotide and amino acid sequences between field isolates and attenuated vaccines. The PCR-RFLP results were identical to those obtained by DNA sequencing and validated their use to differentiate ILTV strains. The approach using the sequencing of the two fragments of the ICP4 gene showed to be an efficient and practical procedure to differentiate between field isolates and vaccine strains of ILTV. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 05/56165-1 - Epidemiologia molecular do vírus da laringotraqueíte infecciosa isolados de surtos em poedeiras comerciais no estado de São Paulo
• Beneficiário: Jorge Luis Chacon Villanueva
• Modalidade de apoio: Bolsas no Brasil - Doutorado