Brassinosteroids interact negatively with jasmonates in the formation of anti-herbivory traits in tomato

Autor(es)

Campos, Marcelo Lattarulo ^[1] ; de Almeida, Marcilio ^[1] ; Rossi, Monica Lanzoni ^[2] ; Martinelli, Adriana Pinheiro ^[2] ; Litholdo Junior, Celso Gaspar ^[2] ; Figueira, Antonio ^[2] ; Rampelotti-Ferreira, Fatima Teresinha ^[3] ; Vendramim, Jose Djair ^[3] ; Benedito, Vagner Augusto ^[2] ; Pereira Peres, Lazaro Eustaquio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Ciencias Biol, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, CENA, BR-13400970 Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Entomol Fitopatol & Entomol Agr, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Experimental Botany; v. 60, n. 15, p. 4346-4360, NOV 2009.
• Citações Web of Science: 80

Resumo

Given the susceptibility of tomato plants to pests, the aim of the present study was to understand how hormones are involved in the formation of tomato natural defences against insect herbivory. Tomato hormone mutants, previously introgressed into the same genetic background of reference, were screened for alterations in trichome densities and allelochemical content. Ethylene, gibberellin, and auxin mutants indirectly showed alteration in trichome density, through effects on epidermal cell area. However, brassinosteroids (BRs) and jasmonates (JAs) directly affected trichome density and allelochemical content, and in an opposite fashion. The BR-deficient mutant dpy showed enhanced pubescence, zingiberene biosynthesis, and proteinase inhibitor expression; the opposite was observed for the JA-insensitive jai1-1 mutant. The dpyxjai1-1 double mutant showed that jai1-1 is epistatic to dpy, indicating that BR acts upstream of the JA signalling pathway. Herbivory tests with the poliphagous insect Spodoptera frugiperda and the tomato pest Tuta absoluta clearly confirmed the importance of the JA-BR interaction in defence against herbivory. The study underscores the importance of hormonal interactions on relevant agricultural traits and raises a novel biological mechanism in tomato that may differ from the BR and JA interaction already suggested for Arabidopsis. (AU)

• Processo FAPESP: 02/00329-8 - Estudo das bases genéticas e bioquímicas da competência para regeneração in vitro utilizando micro tomateiro (Lycopersicon esculentum cv Micro-Tom) como modelo experimental
• Beneficiário: Lázaro Eustaquio Pereira Peres
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 06/05911-8 - Interação entre o ácido jasmônico e outras classes hormonais na resposta à herbivoria em Lycopersicon esculentum Mill. Syn. Solanum lycopersicon L. (Solanaceae)
• Beneficiário: Marcelo Lattarulo Campos
• Linha de fomento: Bolsas no Brasil - Mestrado