Hyperhomocysteinemia induced by feeding rats diets rich in DL-homocysteine thiolactone promotes alterations on carotid reactivity independent of arterial structure

Autor(es)

de Andrade, Claudia Roberta ^[1] ; Tirapelli, Carlos Renato ^[2] ; Haddad, Renato ^[3] ; Eberlin, Marcos N. ^[3] ; Ramalho, Leandra N. Z. ^[4] ; Iyomasa, Mamie M. ^[5] ; Uyemura, Sergio A. ^[6] ; de Oliveira, Ana Maria ^[7]

Afiliação do(s) autor(es)

^[1] USP, FMRP, Dept Pharmacol, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] USP, Dept Psychiat Nursing & Human Sci, Pharmacol Lab, EERP, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[4] USP, FMRP, Dept Pathol, BR-14040903 Ribeirao Preto, SP - Brazil

^[5] USP, FORP, Dept Morphol Sci, BR-14040903 Ribeirao Preto, SP - Brazil

^[6] USP, FCFRP, Dept Clin Toxicol & Food Sci Anal, BR-14040903 Ribeirao Preto, SP - Brazil

^[7] USP, FCFRP, Pharmacol Lab, Dept Chem & Phys, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VASCULAR PHARMACOLOGY; v. 51, n. 4, p. 291-298, OCT 2009.
• Citações Web of Science: 8

Resumo

We aimed to investigate whether Hyperhomocysteinemia (HHcy) interferes on carotid vascular reactivity, and how morphological and functional aspects are related With this put pose male Wistar rats received a Solution Of DL-homocysteine-thiolactone (1 g/kg body weight/day) in tie drinking water for 4, 15 and 30 days. Lipid profile, carotid artery-morphology and -responsiveness to acetylcholine, phenylephrine and endothelin-1 were analyzed Similar increase on homocysteine plasmatic levels occurred in rats treated for 4, 15 and 30 days. High levels of serum cholesterol and triglycerides were observed after HI-Icy 30 days. Vascular reactivity experiments using standard muscle bath procedures showed that HHcy induced a time-dependent reduction on acetylcholine-induced-relaxation at 4, 15 and 30 days. HHcy enhanced the contractile response of endothelium-intact, but not denuded carotid rings to phenylephrine and endothelin-1, despite the treatment time Morphometric analysis showed that intimal/medial area ratio was enhanced only at 30 days of HHcy, despite its reduced cell density The major new finding of the present study is that it establishes a time-course relationship for the events involved on vascular effects associated with HHcy. We demonstrated that alterations on vascular responsiveness precede alterations on arterial structure Based on such findings it is possible to suggest that vascular dysfunction occurs in early stages while alterations on vessel morphology take place in latest stages of HHcy. (C) 2009 Published by Elsevier Inc (AU)

• Processo FAPESP: 01/05549-3 - Consequências da homocisteinemia sobre o sistema endotelina em carótida de ratos: da expressão de receptores à reatividade vascular
• Beneficiário: Claudia Roberta de Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado