Chronic exercise decreases cytokine production in healthy rat skeletal muscle

Autor(es)

Lira, Fabio S. ^{[1, 2]} ; Koyama, Cristiane H. ^[1] ; Yamashita, Alex S. ^[1] ; Rosa, Jose C. ^{[1, 2]} ; Zanchi, Nelo E. ^{[3, 1]} ; Batista, Jr., Miguel L. ^[1] ; Seelaender, Marilia C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Mol Biol Cell Grp, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Physiol, Div Nutr Physiol, BR-05508900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Lab Appl Nutr & Metab, Phys Educ & Sport Sch, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biochemistry and Function; v. 27, n. 7, p. 458-461, OCT 2009.
• Citações Web of Science: 49

Resumo

Skeletal muscle is the source of pro- and anti-inflammatory cytokines, and recently, it has been recognized as an important source of interleukin-6 (IL-6). Acute physical exercise is known to induce a pro-inflammatory cytokine profile in the plasma. However, the effect of chronic physical exercise in the production of pro- and anti-inflammatory cytokines by the skeletal muscle has never been examined. We assessed IL-6, TNF-alpha, IL-1 beta and IL-10 levels in the skeletal muscle of rats submitted to endurance training. Animals were randomly assigned to either a Sedentary group (S, n = 7) or an endurance exercise trained group (T, n = 8). Trained rats ran on a treadmill for 5 days week(-1) for 8 weeks (60% VO(2max)). Detection of IL-6, TNF-alpha, IL-1 beta and IL-10 protein expression was carried out by ELISA. We found decreased expression of IL-1 beta, IL-6, TNF-alpha and IL-10 (28%, 27%. 32% and 37%, respectively, p < 0.05) in the extensor digital longus (EDL) from T, when compared with S. In the soleus, IL-1 beta, TNF-alpha and IL-10 protein levels were similarly decreased (34%, 42% and 50%, respectively, p < 0.05) in T in relation to S, while IL-6 expression was not affected by the training protocol. In conclusion, exercise training induced decreased cytokine protein expression in the skeletal muscle. These data show that in healthy rats, 8-week moderate-intensity aerobic training down regulates skeletal muscle production of cytokines involved in the onset, maintenance and regulation of inflammation, and that the response is heterogeneous according to fibre composition. Copyright (C) 2009 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 05/01445-0 - Papel dos PPARs na caquexia associada ao câncer
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/11588-0 - Expressao de receptores de prostanoides em hepatocitos perioportais e perivenosos: efeitos de mediadores da caquexia associada ao cancer.
• Beneficiário: Marilia Cerqueira Leite Seelaender
• Modalidade de apoio: Auxílio à Pesquisa - Regular